Missotten Guy S O, Notting Irene C, Schlingemann Reinier O, Zijlmans Henry J, Lau Chun, Eilers Paul H C, Keunen Jan E E, Jager Martine J
Departments of Ophthalmology, Pathology, and Medical Statistics, Leiden University Medical Center, Leiden.
Arch Ophthalmol. 2006 Oct;124(10):1428-34. doi: 10.1001/archopht.124.10.1428.
To determine the presence of vascular endothelial growth factor A (VEGF-A) in the aqueous humor of eyes with uveal melanoma and to identify its source.
The VEGF-A concentrations were determined in aqueous humor samples obtained after enucleation from 74 eyes with untreated uveal melanoma and from 8 eyes with treated uveal melanoma. Patient survival and clinical and histopathological tumor variables were compared. In situ hybridization, Western blot analysis, and enzyme-linked immunosorbent assay were used to determine expression of VEGF-A in tumor tissue and in overlying retina.
Aqueous VEGF-A concentrations ranged from 18 to 826 pg/mL in 74 untreated eyes, while concentrations in 30 control eyes were significantly lower (median, 50.1 pg/mL) (P<.001). Concentrations in 8 treated eyes were much higher (median, 364 pg/mL). In situ hybridization on tissue sections and Western blot analysis and enzyme-linked immunosorbent assay on tissue extracts revealed VEGF-A in uveal melanoma tissue and in retinal tissue.
Uveal melanoma is associated with increased concentrations of VEGF-A in aqueous humor. Aqueous VEGF-A concentration correlates with largest basal tumor diameter and with the tumor height. In eyes with uveal melanoma, tumor and retinal tissues are sources of VEGF-A.
确定葡萄膜黑色素瘤患者房水中血管内皮生长因子A（VEGF-A）的存在情况并明确其来源。
测定74只未经治疗的葡萄膜黑色素瘤患眼和8只经治疗的葡萄膜黑色素瘤患眼摘除眼球后获得的房水样本中VEGF-A的浓度。比较患者生存率以及临床和组织病理学肿瘤变量。采用原位杂交、蛋白质印迹分析和酶联免疫吸附测定法来确定VEGF-A在肿瘤组织和覆盖其上的视网膜中的表达情况。
74只未经治疗的患眼房水中VEGF-A浓度范围为18至826 pg/mL，而30只对照眼的浓度显著更低（中位数为50.1 pg/mL）（P<0.001）。8只经治疗的患眼浓度则高得多（中位数为364 pg/mL）。组织切片的原位杂交以及组织提取物的蛋白质印迹分析和酶联免疫吸附测定显示葡萄膜黑色素瘤组织和视网膜组织中存在VEGF-A。
葡萄膜黑色素瘤与房水中VEGF-A浓度升高有关。房水中VEGF-A浓度与最大基底肿瘤直径及肿瘤高度相关。在葡萄膜黑色素瘤患眼中，肿瘤组织和视网膜组织是VEGF-A的来源。
