Kiefer P, Jaques G, Schöneberger J, Heinrich G, Havemann K
Philipps-University, Division of Hematology/Oncology, Marburg, Federal Republic of Germany.
Exp Cell Res. 1991 Feb;192(2):414-7. doi: 10.1016/0014-4827(91)90059-4.
Insulin-like growth factor binding proteins (IGF-BP) are secreted by several human small cell lung cancer cell lines (SCLC). In order to identify the IGF-BPs from SCLC cell lines the RNA from 10 different SCLC cell lines was analyzed by Northern blot analysis with the probes for three different IGF-BPs, IGFBP-1, IGFBP-2, and IGFBP-3. No hybridization signal could be detected with the probes encoding for IGFBP-1 and IGFBP-3. The hybridization with different IGFBP-2-specific oligodeoxynucleotide probes and with the corresponding full-length cDNA showed that all SCLC cell lines which secreted IGF-BPs express IGFBP-2.
胰岛素样生长因子结合蛋白（IGF-BP）由多种人类小细胞肺癌细胞系（SCLC）分泌。为了从小细胞肺癌细胞系中鉴定出IGF-BP，使用针对三种不同IGF-BP（IGFBP-1、IGFBP-2和IGFBP-3）的探针，通过Northern印迹分析对10种不同小细胞肺癌细胞系的RNA进行了分析。编码IGFBP-1和IGFBP-3的探针未检测到杂交信号。与不同的IGFBP-2特异性寡脱氧核苷酸探针以及相应的全长cDNA的杂交表明，所有分泌IGF-BP的小细胞肺癌细胞系均表达IGFBP-2。
