Wegmann B R, Schöneberger H J, Kiefer P E, Jaques G, Brandscheid D, Havemann K
Department of Internal Medicine, Philipps University Marburg, F.R.G.
Eur J Cancer. 1993;29A(11):1578-84. doi: 10.1016/0959-8049(93)90298-t.
We showed recently that insulin-like growth factor binding protein (IGFBP)-1, -2 and -3 are differentially expressed in lung cancer and permanent lung cancer cell lines. Elevated levels of IGF binding capacity in serum of lung cancer patients were also reported. The function and tissue specificity of IGFBP are still obscure but they are probably local regulators of IGF action. Here we show the expression of IGFBP-4 transcripts in 11/11 small cell lung cancer (SCLC) cell lines, in nine out of 11 non-small cell lung cancer (NSCLC) cell lines, in 11/11 lung tumour specimens (10 derived from patients with NSCLC and one from SCLC origin) and in normal lung. In addition we isolated IGFBP-5 cDNA from lambda gt10 libraries of SCLC cell lines. With this IGFBP-5 cDNA we detected transcripts of different lengths in seven out of 11 SCLC cell lines, in 11/11 lung cancer specimens but only in one out of 11 NSCLC cell lines and in normal lung. IGFBP-6 was not detected in northern analysis of any tested SCLC cell line but it was expressed in nine out of 11 NSCLC cell lines and in nine out of 11 human lung cancer specimens and in normal lung.
我们最近发现，胰岛素样生长因子结合蛋白（IGFBP）-1、-2和-3在肺癌及永久性肺癌细胞系中呈差异表达。也有报道称肺癌患者血清中IGF结合能力升高。IGFBP的功能和组织特异性仍不清楚，但它们可能是IGF作用的局部调节因子。在此我们展示了IGFBP-4转录本在11/11个小细胞肺癌（SCLC）细胞系、11个非小细胞肺癌（NSCLC）细胞系中的9个、11/11个肺肿瘤标本（10个来自NSCLC患者，1个来自SCLC）以及正常肺组织中的表达情况。此外，我们从小细胞肺癌细胞系的λgt10文库中分离出了IGFBP-5 cDNA。利用该IGFBP-5 cDNA，我们在11个小细胞肺癌细胞系中的7个、11/11个肺癌标本中检测到了不同长度的转录本，但在11个非小细胞肺癌细胞系中的1个以及正常肺组织中未检测到。在对任何测试的小细胞肺癌细胞系进行Northern分析时均未检测到IGFBP-6，但它在11个非小细胞肺癌细胞系中的9个、11个人类肺癌标本中的9个以及正常肺组织中均有表达。
