Grard Gilda, Lemasson Jean-Jacques, Sylla Massamba, Dubot Audrey, Cook Shelley, Molez Jean-Francois, Pourrut Xavier, Charrel Rémi, Gonzalez Jean-Paul, Munderloh Ulrike, Holmes Edward C, de Lamballerie Xavier
Unité des Virus Emergents (EA3292, IFR48, IRD UR0178), Faculté de Médecine La Timone, 27 boulevard Jean Moulin, 13005 Marseille, France.
IRD - UR0178, Conditions et Territoires d'Emergence des Maladies, BP 1386, 18524 Dakar, Senegal.
J Gen Virol. 2006 Nov;87(Pt 11):3273-3277. doi: 10.1099/vir.0.82071-0.
By using degenerate primers deduced from conserved patterns in the flavivirus polymerase gene, a novel RNA virus was discovered in Rhipicephalus ticks sampled from members of the family Bovidae in Senegal. It was named Ngoye virus (NGOV) after the location from which it was isolated. Viral particles could be observed by electron microscopy, but isolation in vertebrate or invertebrate cell lines or by intracerebral infection of newborn mice remained unsuccessful. This is atypical of recognized arboviruses. The characterization of 4176 nt of the non-structural genes revealed that NGOV is a novel flavivirus species. It forms a distinct phylogenetic lineage related distantly to previously identified members of the genus Flavivirus. Analysis of genetic data suggested that the processing of the NGOV polyprotein and the organization of its replication complex are similar to those of flaviviruses. Together with other recent data, these findings suggest that a large number of viruses related distantly to 'classical' arthropod-borne flaviviruses remain to be discovered.
通过使用从黄病毒属聚合酶基因保守模式推导而来的简并引物，在从塞内加尔牛科动物采集的璃眼蜱中发现了一种新型RNA病毒。它以分离地点命名为恩戈耶病毒（NGOV）。通过电子显微镜可以观察到病毒颗粒，但在脊椎动物或无脊椎动物细胞系中进行分离，或通过新生小鼠脑内感染进行分离均未成功。这对于公认的虫媒病毒来说并不典型。对4176个核苷酸的非结构基因进行表征后发现，NGOV是一种新型黄病毒。它形成了一个与黄病毒属先前确定的成员关系较远的独特系统发育谱系。遗传数据分析表明，NGOV多蛋白的加工及其复制复合体的组织与黄病毒相似。结合其他近期数据，这些发现表明，仍有大量与“经典”虫媒黄病毒关系较远的病毒有待发现。
