肿瘤抗原存在于蜂王浆糖蛋白的N-聚糖中：蜜蜂细胞在N-聚糖部分合成T抗原单位。

Tumor antigen occurs in N-glycan of royal jelly glycoproteins: honeybee cells synthesize T-antigen unit in N-glycan moiety.

作者信息

Kimura Yoshinobu, Ushijima Takayuki, Maeda Megumi, Hama Yoichiro, Kimura Mariko, Okihara Kiyoshi, Sugimoto Hiroyuki, Yamada Hideo

机构信息

Department of Biofunctional Chemistry, Division of Bioscience, Graduate School of Natural Science and Technology, Okayama University, Japan.

出版信息

Biosci Biotechnol Biochem. 2006 Oct;70(10):2583-7. doi: 10.1271/bbb.60331. Epub 2006 Oct 7.

DOI:10.1271/bbb.60331

PMID:17031027

Abstract

In our previous paper (Kimura, Y., et al., Biosci. Biotechnol. Biochem., 67, 1852-1856, 2003), we found that a complex type N-glycans containing beta1-3 galactose residue occurs on royal jelly glycoproteins. During structural analysis of minor components of royal jelly N-glycans, we found complex type N-glycans bearing both galactose and N-acetylgalactosamine residues. Detailed structural analysis of pyridylaminated oligosaccharide revealed that the newly found N-glycan had a complex type structure harboring a tumor marker (T-antigen) unit: Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-6 (Galbeta1-3GalNAcbeta1-4GlcNAcbeta1-2Manalpha1-3) Manbeta1-4GlcNAcbeta1-4GlcNAc. To our knowledge, this may be the first report of the presence of the T-antigen unit in the N-glycan moiety of eucaryotic glycoproteins.

摘要

在我们之前的论文（Kimura, Y.等人，《生物科学、生物技术与生物化学》，67卷，1852 - 1856页，2003年）中，我们发现蜂王浆糖蛋白上存在含有β1-3半乳糖残基的复合型N-聚糖。在对蜂王浆N-聚糖次要成分进行结构分析时，我们发现了同时带有半乳糖和N-乙酰半乳糖胺残基的复合型N-聚糖。对吡啶基氨基化寡糖的详细结构分析表明，新发现的N-聚糖具有包含肿瘤标志物（T-抗原）单元的复合型结构：Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-6（Galβ1-3GalNAcβ1-4GlcNAcβ1-2Manα1-3）Manβ1-4GlcNAcβ1-4GlcNAc。据我们所知，这可能是真核生物糖蛋白N-聚糖部分存在T-抗原单元的首次报道。