Institute of Apicultural Research/Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture and Rural Affairs, Chinese Academy of Agricultural Sciences, Beijing 100093, China.
Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Biomolecules. 2021 Jan 6;11(1):64. doi: 10.3390/biom11010064.
Royal jelly (RJ), a natural honeybee product, has a wide range of antibacterial activities. N-glycosylated major royal jelly protein 2 (N-MRJP2), purified from RJ, can inhibit the growth of (, Gram-positive), a contagious etiological agent of the American foulbrood disease of honeybees. However, the inhibitory mechanism is largely unknown. Antibacterial assay and membrane proteome were conducted to investigate the inhibition capacity of RJ from different instar larvae and treated by N-MRJP2, respectively. The similar antibacterial efficiency of RJ from different larval instar indicates that RJ is vital for the adaptive immune defense of small larvae. The killing of by N-MRJP2 is achieved by disturbing the cell wall biosynthesis, increasing the permeability of cell membrane, hindering aerobic respiration, restraining cell division and inducing cell death. This demonstrates that RJ is critical for the passive immunity of immature larvae and N-MRJP2 can be used as natural antibiotic substance to resist , even for other gram-positive bacteria. This constitutes solid evidence that RJ and N-MRJP2 have potentials as novel antibacterial agents.
蜂王浆（RJ）是一种天然的蜜蜂产品，具有广泛的抗菌活性。从 RJ 中纯化得到的 N-糖基化主要蜂王浆蛋白 2（N-MRJP2）可以抑制革兰氏阳性菌的生长，这是一种传染性的蜜蜂美洲幼虫腐臭病的病原体。然而，其抑制机制在很大程度上尚不清楚。本研究分别通过抗菌测定和膜蛋白质组学分析，研究了来自不同龄期幼虫的 RJ 和经 N-MRJP2 处理的 RJ 的抑制能力。来自不同龄期幼虫的 RJ 具有相似的抗菌效率，这表明 RJ 对于小幼虫的适应性免疫防御至关重要。N-MRJP2 通过干扰细胞壁生物合成、增加细胞膜通透性、阻碍需氧呼吸、抑制细胞分裂和诱导细胞死亡来杀死 。这表明 RJ 对于未成熟幼虫的被动免疫至关重要，并且 N-MRJP2 可以用作天然抗生素物质来抵抗 ，甚至可以抵抗其他革兰氏阳性菌。这为 RJ 和 N-MRJP2 作为新型抗菌剂具有潜力提供了确凿的证据。
