Van den Beucken J J J P, Walboomers X F, Vos M R J, Sommerdijk N A J M, Nolte R J M, Jansen J A
Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Center, PO Box 9101, Nijmegen 6500 HB, the Netherlands.
J Biomed Mater Res A. 2007 Mar 1;80(3):612-20. doi: 10.1002/jbm.a.30971.
A pivotal factor to consider in the development of biomaterials and biomaterial coatings is the inflammatory response to these materials. The insertion of implants is followed by protein adsorption and subsequent interactions with cellular components of the biological surroundings, in which macrophages play a dominant role through the production of a myriad of signaling molecules. In view of this, the aims of the present study were to evaluate (i) gross protein adsorption to, and (ii) in vitro behavior of macrophages on novel biomaterial coatings, composed of poly-D-lysine (PDL) or poly(allylamine hydrochloride) (PAH) and DNA, and to compare these coatings with negative (noncoated glass) and positive controls (noncoated glass + LPS-stimulation). The results demonstrate that multilayered DNA-coatings do not affect gross protein adsorption compared to noncoated controls. Cell culture experiments showed that the attachment to, and viability and morphology of two types of macrophages cultured on multilayered DNA-coatings is comparable to noncoated controls. Still, macrophages repeatedly showed decreased secretion levels of the proinflammatory cytokine TNF-alpha on multilayered DNA-coatings, whereas no differences were observed in the secretion of IL-1beta, IL-10, and TGF-beta1. Appropriate animal studies are required to elucidate if these in vitro indications have clinical effects on the inflammatory and wound healing processes around implants.
在生物材料和生物材料涂层的研发过程中，一个关键的考虑因素是这些材料所引发的炎症反应。植入物植入后会发生蛋白质吸附，随后与生物环境中的细胞成分相互作用，其中巨噬细胞通过产生大量信号分子发挥主导作用。鉴于此，本研究的目的是评估：（i）新型生物材料涂层（由聚-D-赖氨酸（PDL）或聚（烯丙胺盐酸盐）（PAH）与DNA组成）上的总蛋白质吸附情况；（ii）巨噬细胞在这些涂层上的体外行为，并将这些涂层与阴性对照（未涂层玻璃）和阳性对照（未涂层玻璃+脂多糖刺激）进行比较。结果表明，与未涂层对照相比，多层DNA涂层不会影响总蛋白质吸附。细胞培养实验表明，在多层DNA涂层上培养的两种巨噬细胞的附着、活力和形态与未涂层对照相当。不过，巨噬细胞在多层DNA涂层上反复表现出促炎细胞因子TNF-α的分泌水平降低，而在IL-1β、IL-10和TGF-β1的分泌方面未观察到差异。需要进行适当的动物研究，以阐明这些体外指标是否对植入物周围的炎症和伤口愈合过程具有临床影响。
