Chamberlain Lisa M, Godek Marisha L, Gonzalez-Juarrero Mercedes, Grainger David W
Cell and Molecular Biology Program, Colorado State University, Fort Collins, Colorado 80523, USA.
J Biomed Mater Res A. 2009 Mar 15;88(4):858-71. doi: 10.1002/jbm.a.31930.
Cells of the mononuclear phagocytic system including monocytes and macrophages (e.g., pooled human monocytes, bone marrow-derived macrophages, etc.) are often employed for in vitro assessment of novel biomaterials and to assay anti-inflammatory drug activity. In this context, numerous macrophage cells are treated interchangeably in the literature despite a lack of demonstrated equivalence among immortalized cell lines and further, between cell lines and primary-derived macrophages of different species. Three murine (monocyte-) macrophage cell lines (IC-21, J774A.1, and RAW 264.7), commonly utilizedin biomaterial and pharmaceutical screening research, have been compared with primary-derived murine bone marrow macrophages. Significant differences were discovered in the expression of cell surface proteins requisite for cell adhesion and activation among cell lines and primary-derived cells as well as between the different cell lines. Results demonstrate activation but with reduced cytokine expression to chemical stimulus (lipopolysaccharide) by cell lines compared with that of primary-derived macrophages. Limited correlation between cultured primary and immortalized cells in cytokine production, phenotype and intrinsic activation states has relevance to fidelity for in vitro testing. These differences warrant justification for selection of various cell lines for specific assay purposes, and merit caution if comparisons to primary cell types (i.e., for biocompatibility) are required.
单核吞噬细胞系统的细胞，包括单核细胞和巨噬细胞（例如，汇集的人单核细胞、骨髓来源的巨噬细胞等），常被用于新型生物材料的体外评估以及抗炎药物活性的测定。在此背景下，尽管永生化细胞系之间以及不同物种的细胞系与原代巨噬细胞之间缺乏已证实的等效性，但文献中仍常常对众多巨噬细胞进行互换处理。三种常用于生物材料和药物筛选研究的小鼠（单核细胞 -）巨噬细胞系（IC - 21、J774A.1和RAW 264.7）已与原代小鼠骨髓巨噬细胞进行了比较。在细胞系与原代细胞之间以及不同细胞系之间，发现了细胞黏附和激活所需的细胞表面蛋白表达存在显著差异。结果表明，与原代巨噬细胞相比，细胞系对化学刺激（脂多糖）有激活反应，但细胞因子表达降低。原代培养细胞与永生化细胞在细胞因子产生、表型和内在激活状态方面的相关性有限，这与体外测试的保真度有关。这些差异为针对特定测定目的选择各种细胞系提供了依据，如果需要与原代细胞类型进行比较（例如用于生物相容性），则需谨慎。
