Halperin S A, Issekutz T B, Kasina A
Department of Pediatrics, Dalhousie University, Halifax, Canada.
J Infect Dis. 1991 Feb;163(2):355-61. doi: 10.1093/infdis/163.2.355.
Three monoclonal antibodies to pertussis toxin were characterized and used to investigate its role in immunity. Antibody affinity correlated with toxin neutralization in in vivo and in vitro assays but was not the only determinant of protection against Bordetella pertussis infection. B9, a high-affinity anti-S3 antibody, was the most effective in neutralizing toxin-induced CHO cell clustering and hemagglutination in vitro and lymphocytosis and histamine sensitization in vivo. A4, a similar-affinity anti-S1 antibody, was less active in the toxin neutralization assays but more protective in the mouse infection model. A12, a low-affinity anti-S1 antibody, was least active in the assays of toxin neutralization but as effective as B9 in the infection model. These data suggest that epitopes on the A protomer and B oligomer may induce protective immunity. Measurement of pertussis toxin neutralization by monoclonal antibodies in in vitro and in vivo assays may not accurately predict protection against infection with B. pertussis.
对三种抗百日咳毒素单克隆抗体进行了特性鉴定，并用于研究其在免疫中的作用。在体内和体外试验中，抗体亲和力与毒素中和作用相关，但不是预防百日咳博德特氏菌感染的唯一决定因素。B9是一种高亲和力抗S3抗体，在体外中和毒素诱导的CHO细胞聚集和血凝以及在体内中和淋巴细胞增多和组胺致敏方面最有效。A4是一种亲和力相似的抗S1抗体，在毒素中和试验中活性较低，但在小鼠感染模型中更具保护作用。A12是一种低亲和力抗S1抗体，在毒素中和试验中活性最低，但在感染模型中与B9一样有效。这些数据表明，A原体和B寡聚体上的表位可能诱导保护性免疫。在体外和体内试验中用单克隆抗体测量百日咳毒素中和作用可能无法准确预测对百日咳博德特氏菌感染的预防作用。
