Kaslow H R, Schlotterbeck J D, Gotto J
Department of Physiology and Biophysics, University of Southern California School of Medicine, Los Angeles 90033.
Dev Biol Stand. 1991;73:143-50.
The monoclonal antibody termed 1B7 neutralizes pertussis toxin in vivo in cell culture systems and can also passively protect mice from a challenge with live Bordetella pertussis (9). It has been suggested that most other independently derived neutralizing monoclonal antibodies recognizing the S1 subunit apparently recognize the same epitope as 1B7, and that the S1 subunit contains only one immunodominant protective epitope (1). These antibodies have been termed Class A antibodies (8) and inhibit the ADP-ribosyltransferase but not the NAD glycohydrolase activity of the toxin (7). We are testing the hypothesis that immunization with inactivated preparations of pertussis toxin that lead to protection are associated with the production of Class A antibodies. If true, then identification of Class A antibodies in sera might provide a serological correlate of protection. If false, then development of assays designed to detect the important protective antibodies are necessary. Our initial results suggest that Class A antibodies are not the predominant neutralizing antibody in mice immunized with vaccines containing formalin-treated pertussis toxin.
名为1B7的单克隆抗体在细胞培养系统中可在体内中和百日咳毒素，还能被动保护小鼠免受活的百日咳博德特氏菌的攻击（9）。有人提出，大多数其他独立产生的识别S1亚基的中和性单克隆抗体显然与1B7识别相同的表位，并且S1亚基仅包含一个免疫显性保护性表位（1）。这些抗体被称为A类抗体（8），可抑制毒素的ADP-核糖基转移酶活性，但不抑制其NAD糖水解酶活性（7）。我们正在检验这样一个假设，即导致产生保护作用的百日咳毒素灭活制剂免疫接种与A类抗体的产生有关。如果这一假设成立，那么血清中A类抗体的鉴定可能提供保护作用的血清学关联指标。如果假设不成立，那么就有必要开发旨在检测重要保护性抗体的检测方法。我们的初步结果表明，在用含有经福尔马林处理的百日咳毒素的疫苗免疫的小鼠中，A类抗体并非主要的中和抗体。
