一种比率型CdSe/ZnS纳米晶体pH传感器。

A ratiometric CdSe/ZnS nanocrystal pH sensor.

作者信息

Snee Preston T, Somers Rebecca C, Nair Gautham, Zimmer John P, Bawendi Moungi G, Nocera Daniel G

机构信息

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139-4306, USA.

出版信息

J Am Chem Soc. 2006 Oct 18;128(41):13320-1. doi: 10.1021/ja0618999.

DOI:10.1021/ja0618999

PMID:17031920

Abstract

The development of a reversible chemical sensor based on a CdSe/ZnS nanocrystal (NC) is described. Signal transduction is accomplished by fluorescence resonance energy transfer (FRET) between the NC and a fluorescent pH-sensitive squaraine dye attached to the surface of the NC. The efficiency of FRET, and consequently the relative intensity of NC and dye emissions, is modulated with the pH-dependent absorption cross section of the squaraine dye. The design of a NC sensor based on FRET results in a ratiometric sensor since the emission intensities of dye and NC may be referenced to the isosbestic point between NC and dye emissions. The ratiometric approach allows sensing to be performed, regardless of issues surrounding collection efficiency (scattering environment, light fluctuations, etc.) and dye:NC loadings.

摘要

描述了一种基于CdSe/ZnS纳米晶体（NC）的可逆化学传感器的开发。信号转导通过NC与附着在NC表面的荧光pH敏感方酸染料之间的荧光共振能量转移（FRET）来实现。FRET的效率以及因此NC和染料发射的相对强度，由方酸染料的pH依赖性吸收截面调节。基于FRET的NC传感器设计产生了一种比率传感器，因为染料和NC的发射强度可以参考NC和染料发射之间的等吸收点。比率方法允许进行传感，而无需考虑收集效率（散射环境、光波动等）和染料：NC负载等问题。