Wang Q, Li H, Li N, Leng L, Wang Y
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Poult Sci. 2006 Nov;85(11):1890-5. doi: 10.1093/ps/85.11.1890.
Fatty acid-binding proteins belong to a superfamily of lipid-binding proteins that exhibit a high affinity for long-chain fatty acids and appear to function in metabolism and intracellular transportation of lipids. The current study was designed to investigate expression characterization and association with growth and composition traits of the liver fatty acid-binding protein (L-FABP) gene in the chicken. Northern blot analysis indicated that the gene, similar to the mammal L-FABP gene, was expressed only in liver and intestinal tissues. The mRNA levels of the chicken L-FABP gene in liver and intestine had significant differences between the broilers and Baier layers. The China Agricultural University F(2) population was used in the present study. Body weight and body composition traits were measured in the populations. Primers for the coding region and 5' upstream region of the L-FABP gene were designed according to chicken genomic and cDNA sequence. Polymorphisms were detected by DNA sequencing, and the PCR single-strand conformation polymorphisms method was developed to genotype the F(2) population. The results indicated that the L-FABP gene polymorphisms were associated with abdominal fat weight and percentage of abdominal fat, and the L-FABP gene could be a candidate locus or linked to a major gene(s) that affects fatness traits in the chicken. The results of the current study provided basic molecular information for studying the role of the L-FABP gene in the regulation of lipid metabolism in avian species.
脂肪酸结合蛋白属于脂质结合蛋白超家族，对长链脂肪酸具有高亲和力，似乎在脂质的代谢和细胞内运输中发挥作用。本研究旨在调查鸡肝脏脂肪酸结合蛋白（L-FABP）基因的表达特征及其与生长和体组成性状的关联。Northern印迹分析表明，该基因与哺乳动物L-FABP基因相似，仅在肝脏和肠道组织中表达。肉鸡和白耳鸡肝脏和肠道中鸡L-FABP基因的mRNA水平存在显著差异。本研究使用了中国农业大学的F2群体。对该群体测量了体重和体组成性状。根据鸡的基因组和cDNA序列设计了L-FABP基因编码区和5'上游区的引物。通过DNA测序检测多态性，并开发了PCR单链构象多态性方法对F2群体进行基因分型。结果表明，L-FABP基因多态性与腹脂重量和腹脂百分比相关，L-FABP基因可能是影响鸡脂肪性状的一个候选基因座或与一个主基因连锁。本研究结果为研究L-FABP基因在禽类脂质代谢调控中的作用提供了基础分子信息。
