Zheng Aijuan, Chang Wenhuan, Liu Guohua, Yue Ying, Li Jianke, Zhang Shu, Cai Huiyi, Yang Aijun, Chen Zhimin
Feed Research Institute/Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Key Laboratory of Pollinating Insect Biology of Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
PLoS One. 2016 Oct 19;11(10):e0164702. doi: 10.1371/journal.pone.0164702. eCollection 2016.
Identifying the metabolic differences in the livers of modern broilers and local chicken breeds is important for understanding their biological characteristics, and many proteomic changes in their livers are not well characterized. We therefore analyzed the hepatic protein profiles of a commercial breed, Arbor Acres (AA) broilers, and a local dual purpose breed, Big Bone chickens, using two-dimensional electrophoresis combined with liquid chromatography-chip/electrospray ionization-quadruple time-of-flight/mass spectrometry (LC-MS/MS). A total of 145 proteins were identified as having differential abundance in the two breeds at three growth stages. Among them, 49, 63 and 54 belonged to 2, 4, and 6 weeks of age, respectively. The higher abundance proteins in AA broilers were related to the energy production pathways suggesting enhanced energy metabolism and lipid biosynthesis. In contrast, the higher abundance proteins in Big Bone chickens showed enhanced lipid degradation, resulting in a reduction in the abdominal fat percentage. Along with the decrease in fat deposition, flavor substance synthesis in the meat of the Big Bone chickens may be improved by enhanced abundance of proteins involved in glycine metabolism. In addition, the identified proteins in nucleotide metabolism, antioxidants, cell structure, protein folding and transporters may be critically important for immune defense, gene transcription and other biological processes in the two breeds. These results indicate that selection pressure may have shaped the two lines differently resulting in different hepatic metabolic capacities and extensive metabolic differences in the liver. The results from this study may help provide the theoretical basis for chicken breeding.
识别现代肉鸡和本地鸡品种肝脏中的代谢差异对于了解它们的生物学特性很重要，并且它们肝脏中的许多蛋白质组学变化尚未得到充分表征。因此，我们使用二维电泳结合液相色谱芯片/电喷雾电离-四极杆飞行时间/质谱（LC-MS/MS）分析了商业品种爱拔益加（AA）肉鸡和本地两用品种大骨鸡的肝脏蛋白质谱。在三个生长阶段，共鉴定出145种在两个品种中丰度有差异的蛋白质。其中，49种、63种和54种分别属于2周龄、4周龄和6周龄。AA肉鸡中丰度较高的蛋白质与能量产生途径有关，表明能量代谢和脂质生物合成增强。相比之下，大骨鸡中丰度较高的蛋白质显示脂质降解增强，导致腹部脂肪百分比降低。随着脂肪沉积的减少，大骨鸡肉中风味物质的合成可能通过参与甘氨酸代谢的蛋白质丰度增加而得到改善。此外，在核苷酸代谢、抗氧化剂、细胞结构、蛋白质折叠和转运蛋白中鉴定出的蛋白质可能对两个品种的免疫防御、基因转录和其他生物学过程至关重要。这些结果表明，选择压力可能以不同方式塑造了这两个品系，导致它们肝脏的代谢能力不同以及肝脏中存在广泛的代谢差异。本研究结果可能有助于为鸡的育种提供理论依据。
