Javaherian K, Langlois A J, LaRosa G J, Profy A T, Bolognesi D P, Herlihy W C, Putney S D, Matthews T J
Department of Surgery, Duke University Medical School, Durham, NC 27710.
Science. 1990 Dec 14;250(4987):1590-3. doi: 10.1126/science.1703322.
The principal neutralizing determinant (PND) of human immunodeficiency virus (HIV)-1 resides within the V3 loop of the envelope protein. Antibodies elicited by peptides of this region were able to neutralize diverse isolates. Serum from one of three animals immunized with the human T cell lymphoma virus (HTLV)-IIIMN PND peptide, RP142, neutralized MN and the sequence-divergent HTLV-IIIB isolate. Serum from one of three animals immunized with a 13-amino acid IIIB PND peptide (RP337) also neutralized both of these isolates. Characterization of these sera revealed that the cross-neutralizing antibodies bound the amino acid sequence GlyProGlyArgAlaPhe (GPGRAF) that is present in both isolates. This sequence is frequently found in the PNDs analyzed in randomly selected HIV-1 isolates. Sera from two rabbits immunized with a peptide containing only the GPGRAF residues neutralized divergent isolates, including IIIB and MN.
人类免疫缺陷病毒1型（HIV-1）的主要中和决定簇（PND）位于包膜蛋白的V3环内。该区域肽段引发的抗体能够中和多种分离株。用人类T细胞淋巴瘤病毒（HTLV）-IIIMN PND肽RP142免疫的三只动物中，有一只动物的血清能中和MN以及序列不同的HTLV-IIIB分离株。用一种13个氨基酸的IIIB PND肽（RP337）免疫的三只动物中，有一只动物的血清也能中和这两种分离株。对这些血清的特性分析表明，交叉中和抗体结合了两种分离株中都存在的氨基酸序列甘氨酸-脯氨酸-甘氨酸-精氨酸-丙氨酸-苯丙氨酸（GPGRAF）。在随机选择的HIV-1分离株中分析的PND中经常发现该序列。用仅含GPGRAF残基的肽免疫的两只兔子的血清能中和包括IIIB和MN在内的不同分离株。
