用生物素化的YAG:Ce3+纳米晶荧光粉标记抗生物素蛋白固定化微珠。

Tagging of avidin immobilized beads with biotinylated YAG:Ce3+ nanocrystal phosphor.

作者信息

Asakura Ryo, Isobe Tetsuhiko, Kurokawa Kiyoshi, Aizawa Hideki, Ohkubo Michio

机构信息

Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, 223-8522, Japan.

出版信息

Anal Bioanal Chem. 2006 Nov;386(6):1641-7. doi: 10.1007/s00216-006-0814-6. Epub 2006 Oct 11.

DOI:10.1007/s00216-006-0814-6

PMID:17033769

Abstract

YAG

Ce3+ nanoparticles 9.5+/-1.2 nm in diameter have been synthesized from aluminium isopropoxide and acetates of yttrium and cerium in 1,4-butanediol (1,4-BD) by autoclave treatment at 300 degrees C for 2 h. After replacing 1,4-BD by ultrapure water, NH2 groups were introduced on the surface of YAG:Ce3+ nanoparticles by addition of 3-aminopropyltrimethoxysilane then biotinylation with sulfo-NHS-LC-biotin. We demonstrated that avidin immobilized beads are tagged by biotinylated YAG:Ce3+ nanoparticles by the selective avidin-biotin interaction, furnishing a green fluorescent image on excitation with blue light. This result indicates that YAG:Ce3+ nanoparticle phosphors have much potential in biological applications.

摘要

通过在300℃下用高压釜处理2小时，由异丙醇铝以及钇和铈的醋酸盐在1,4-丁二醇（1,4-BD）中合成了直径为9.5±1.2nm的YAG:Ce3+纳米颗粒。在用超纯水取代1,4-BD后，通过添加3-氨丙基三甲氧基硅烷在YAG:Ce3+纳米颗粒表面引入NH2基团，然后用磺基-NHS-LC-生物素进行生物素化。我们证明，通过选择性抗生物素蛋白-生物素相互作用，固定有抗生物素蛋白的珠子被生物素化的YAG:Ce3+纳米颗粒标记，在蓝光激发下提供绿色荧光图像。该结果表明YAG:Ce3+纳米颗粒荧光粉在生物应用中具有很大潜力。