Blanco Antonio M, Moreno José, Del Campo José A, Rivas Joaquín, Guerrero Miguel G
Instituto de Bioquímica Vegetal y Fotosíntesis, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Avda. Américo Vespucio 49, Sevilla, 41092, Spain.
Appl Microbiol Biotechnol. 2007 Jan;73(6):1259-66. doi: 10.1007/s00253-006-0598-9. Epub 2006 Oct 11.
The growth performance of the chlorophycean microalga Muriellopsis sp. outdoors in open tanks agitated with a paddlewheel and its ability to accumulate carotenoids have been evaluated throughout the year. The cells grown in the open system had free lutein as the main carotenoid, with violaxanthin, beta-carotene, and neoxanthin also present. Lutein content of the dry biomass ranged from 0.4 to 0.6%, depending on the growth and environmental conditions. In addition, the biomass of Muriellopsis sp. had a high content in both protein and lipids with about half of the fatty acids being of the polyunsaturated type, with alpha-linolenic acid accounting for almost 30% of the total fatty acids. The effect of determinant parameters on the performance of the cultures in open tanks was evaluated. Operating conditions that allow the maintenance of productive cultures were established under semicontinuous regime for 9 months throughout the year. Biomass and lutein yields in the open system were not far from those in closed tubular photobioreactors, and reached productivity values of 20 g dry biomass, containing around 100 mg lutein m(-2) day(-1) in summer. The outdoor culture of Muriellopsis sp. in open ponds thus represents a real alternative to established systems for the production of lutein.
对绿藻门微藻穆氏藻（Muriellopsis sp.）在户外敞口池中用桨轮搅拌培养时的生长性能及其积累类胡萝卜素的能力进行了全年评估。在开放系统中生长的细胞以游离叶黄素作为主要类胡萝卜素，同时还存在紫黄质、β-胡萝卜素和新黄质。根据生长和环境条件，干生物质中叶黄素含量在0.4%至0.6%之间。此外，穆氏藻的生物质在蛋白质和脂质方面含量都很高，约一半的脂肪酸为多不饱和类型，其中α-亚麻酸占总脂肪酸的近30%。评估了决定性参数对敞口池中培养物性能的影响。全年在半连续模式下建立了能维持高产培养物的操作条件，为期9个月。开放系统中的生物质和叶黄素产量与封闭管式光生物反应器中的产量相差不大，夏季达到了20克干生物质的生产力值，每平方米每天含有约100毫克叶黄素。因此，穆氏藻在户外敞口池塘中的培养是叶黄素生产现有系统的一种切实可行的替代方案。
