Mackiewicz Jerzy, Maaijwee Kristel, Lüke Christoph, Kociok Norbert, Hiebl Wilfried, Meinert Hasso, Joussen Antonia M
Department of Vitreoretinal Surgery, Center for Ophthalmology, University of Cologne, Kerpener Strasse 62, 50924, Cologne, Germany.
Graefes Arch Clin Exp Ophthalmol. 2007 May;245(5):665-75. doi: 10.1007/s00417-006-0414-3. Epub 2006 Oct 11.
In order to investigate whether gravity is the reason for retinal degeneration in long-term vitreous tamponade, perfluorohexyloctane (F6H8), perfluorodecalin (PFD), and a mixture of F6H8/PFD were compared.
Each group of 5 rabbits received a 3-month tamponade with either PFD (pure) (1.93 g/cm(3)), F6H8 (pure) (1.33 g/cm(3)), or a 1:1 mixture of F6H8/PFD (1.62 g/cm(3)). Electroretinograms (ERG) were performed pre- and postoperatively. Lower and upper retinal areas were compared using immunohistochemical methods. Transmission electron microscopy was performed to investigate alterations in the photoreceptors.
All three substances were tolerated well in rabbit eyes for up to 3 months. Dispersion was seen earliest with PFD and latest with pure F6H8. None of the substances demonstrated inflammatory reactions or vascular alterations. ERGs were not considerably altered with any of the substances. Histology of the retina showed alterations in the cell counts within the inner and outer nuclear layer that were not attributable to the gravity of the tamponading agent.
In contrast to previously published work, this study did not detect any tamponade-related structural damage of the retina after a 3-months tamponade in the rabbit model. Based upon this study, we conclude that gravity might not be causally linked to retinal damage.
为了研究重力是否是长期玻璃体填塞导致视网膜变性的原因，对全氟己基辛烷（F6H8）、全氟萘烷（PFD）以及F6H8/PFD混合物进行了比较。
每组5只兔子，分别接受为期3个月的PFD（纯品）（1.93 g/cm³）、F6H8（纯品）（1.33 g/cm³）或F6H8/PFD 1:1混合物（1.62 g/cm³）填塞。在术前和术后进行视网膜电图（ERG）检查。使用免疫组织化学方法比较视网膜上下区域。进行透射电子显微镜检查以研究光感受器的变化。
所有三种物质在兔眼中长达3个月的时间内耐受性良好。PFD最早出现弥散，纯F6H8最晚出现。所有物质均未显示炎症反应或血管改变。使用任何一种物质后ERG均未发生明显改变。视网膜组织学显示内核层和外核层的细胞计数有变化，但这并非由填塞剂的重力所致。
与先前发表的研究结果不同，本研究在兔模型中进行3个月的填塞后未检测到任何与填塞相关的视网膜结构损伤。基于本研究，我们得出结论，重力可能与视网膜损伤没有因果关系。
