Luff Jennifer A, Burns Rachel E, Mader Michelle, Priest Kara D, Tuttle Allison D
Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC (Luff, Mader).
Department of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, University of Connecticut, Storrs, CT (Burns, Priest).
J Vet Diagn Invest. 2018 Jul;30(4):572-575. doi: 10.1177/1040638718769702. Epub 2018 Apr 9.
Papillomaviruses (PVs) are found in many species and infect epithelial cells at both mucosal and cutaneous sites. PVs are generally species-specific and cause benign epithelial proliferations, often forming papillomas or plaques. Rarely, these infections can persist, allowing progression to in situ and invasive cancers. We describe herein a case of multiple cutaneous pigmented plaques from a California sea lion ( Zalophus californianus) that progressed to in situ and invasive squamous cell carcinoma (SCC). The lesions were characterized by epithelial hyperplasia, hyperkeratosis, and hypergranulosis that bordered more dysplastic areas, and, at one site, bordered an invasive SCC. Immunohistochemistry for papillomavirus antigen revealed strong nuclear immunoreactivity within keratinocytes in the hyperplastic epidermis. PCR was performed using degenerate and specific primers to detect papillomavirus DNA. Specific primers were used to amplify Zalophus californianus papillomavirus 1 (ZcPV-1), the only sea lion papillomavirus known to date. We detected ZcPV-1 DNA within the pigmented plaque, and in both in situ and invasive SCC samples.
乳头瘤病毒（PVs）存在于许多物种中，可感染黏膜和皮肤部位的上皮细胞。PVs通常具有物种特异性，会引起良性上皮增生，常形成乳头状瘤或斑块。这些感染很少会持续存在，从而进展为原位癌和浸润性癌。我们在此描述一例来自加利福尼亚海狮（Zalophus californianus）的多发性皮肤色素沉着斑块病例，该病例进展为原位和浸润性鳞状细胞癌（SCC）。病变的特征为上皮增生、角化过度和颗粒层增厚，这些区域与发育异常更明显的区域相邻，并且在一个部位与浸润性SCC相邻。乳头瘤病毒抗原的免疫组织化学显示增生表皮的角质形成细胞内有强烈的核免疫反应性。使用简并引物和特异性引物进行聚合酶链反应（PCR）以检测乳头瘤病毒DNA。特异性引物用于扩增加利福尼亚海狮乳头瘤病毒1（ZcPV-1），这是迄今为止已知的唯一一种海狮乳头瘤病毒。我们在色素沉着斑块以及原位和浸润性SCC样本中均检测到了ZcPV-1 DNA。
