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在未成熟T细胞上，βT细胞受体（TCR）链在没有其他TCR或CD3蛋白的情况下的表面表达。

Surface expression of the beta T cell receptor (TCR) chain in the absence of other TCR or CD3 proteins on immature T cells.

作者信息

Kishi H, Borgulya P, Scott B, Karjalainen K, Traunecker A, Kaufman J, von Boehmer H

机构信息

Basel Institute for Immunology, Switzerland.

出版信息

EMBO J. 1991 Jan;10(1):93-100. doi: 10.1002/j.1460-2075.1991.tb07924.x.

DOI:10.1002/j.1460-2075.1991.tb07924.x
PMID:1703490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC452616/
Abstract

T cell receptor (TCR) beta genes are rearranged prior to TCR alpha genes. A productively rearranged TCR beta gene suppresses further V beta gene rearrangement. Here we show that in beta TCR transgenic mice the TCR beta-chain can be expressed on the surface of immature CD4-8- thymocytes, but not on mature T cells, in the absence of any other known TCR chain and proteins of the CD3 complex. Analysis by NEPHGE and SDS-PAGE showed that at least some beta TCR exists on the surface as a large disulfide-linked complex with unknown acidic molecules. The introduction of the beta TCR gene into scid mice resulted in the expression of the beta TCR on the cell surface of thymocytes and induced the expression of CD4 and CD8 co-receptors as well as transcription of the alpha TCR locus.

摘要

T细胞受体（TCR）β基因在TCRα基因之前重排。一个有效重排的TCRβ基因会抑制进一步的Vβ基因重排。我们在此表明，在βTCR转基因小鼠中，在没有任何其他已知TCR链和CD3复合物蛋白的情况下，TCRβ链可在未成熟的CD4-8-胸腺细胞表面表达，但在成熟T细胞表面则不能表达。通过非平衡pH梯度聚丙烯酰胺凝胶电泳（NEPHGE）和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析表明，至少一些βTCR以与未知酸性分子形成的大的二硫键连接复合物的形式存在于细胞表面。将βTCR基因导入严重联合免疫缺陷（scid）小鼠中，导致βTCR在胸腺细胞表面表达，并诱导CD4和CD8共受体的表达以及αTCR基因座的转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/4b42cd7edf5d/emboj00099-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/7742df4d98ce/emboj00099-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/c64cb004bf25/emboj00099-0092-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/9b4a530a7a38/emboj00099-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/44cee2ca58f9/emboj00099-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/a142ac45f7b3/emboj00099-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/7f0c3358cd5a/emboj00099-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/4b42cd7edf5d/emboj00099-0096-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/7742df4d98ce/emboj00099-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/c64cb004bf25/emboj00099-0092-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/9b4a530a7a38/emboj00099-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/44cee2ca58f9/emboj00099-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/a142ac45f7b3/emboj00099-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/7f0c3358cd5a/emboj00099-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe69/452616/4b42cd7edf5d/emboj00099-0096-a.jpg

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