Giustarini Daniela, Milzani Aldo, Dalle-Donne Isabella, Rossi Ranieri
Department of Neuroscience, Pharmacology Section, Via A. Moro 4, University of Siena, 53100 Siena, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2007 May 15;851(1-2):124-39. doi: 10.1016/j.jchromb.2006.09.031. Epub 2006 Oct 10.
Many different methodologies have been applied for the detection of S-nitrosothiols (RSNOs) in human biological fluids. One unsatisfactory outcome of the last 14 years of research focused on this issue is that a general consensus on reference values for physiological RSNO concentration in human blood is still missing. Consequently, both RSNO physiological function and their role in disease have not yet been clarified. Here, a summary of the values measured for RSNOs in erythrocytes, plasma, and other biological fluids is provided, together with a critical review of the most widely used analytical methods. Furthermore, some possible methodological drawbacks, responsible for the highlighted discrepancies, are evidenced.
许多不同的方法已被应用于检测人体生物体液中的S-亚硝基硫醇（RSNOs）。过去14年专注于这个问题的研究的一个不尽人意的结果是，对于人体血液中生理RSNO浓度的参考值仍未达成普遍共识。因此，RSNO的生理功能及其在疾病中的作用尚未得到阐明。在此，提供了红细胞、血浆和其他生物体液中RSNOs测量值的总结，以及对最广泛使用的分析方法的批判性综述。此外，还指出了一些导致显著差异的可能的方法学缺陷。
