Teng Xinjun, Scott Isbell T, Crawford Jack H, Bosworth Charles A, Giles Gregory I, Koenitzer Jeffrey R, Lancaster Jack R, Doeller Jeannette E, W Kraus David, P Patel Rakesh
Department of Pathology, University of Alabama at Birmingham, AL, USA.
Methods Enzymol. 2008;441:161-72. doi: 10.1016/S0076-6879(08)01209-3.
Recent advances in techniques that allow sensitive and specific measurement of S-nitrosothiols (RSNOs) have provided evidence for a role for these compounds in various aspects of nitric oxide (NO) biology. The most widely used approach is to couple reaction chemistry that selectively reduces RSNOs by one electron to produce NO, with the sensitive detection of the latter under anaerobic conditions using ozone based chemiluminescence in NO analyzers. Herein, we report a novel reaction that is readily adaptable for commercial NO analyzers that utilizes hydrogen sulfide (H2S), a gas that can reduce RSNO to NO and, analogous to NO, is produced by endogenous metabolism and has effects on diverse biological functions. We discuss factors that affect H2S based methods for RSNO measurement and discuss the potential of H2S as an experimental tool to measure RSNO.
能够对S-亚硝基硫醇（RSNOs）进行灵敏且特异测量的技术的最新进展，为这些化合物在一氧化氮（NO）生物学各个方面所起的作用提供了证据。最广泛使用的方法是将通过单电子选择性还原RSNOs以产生NO的反应化学，与在厌氧条件下使用NO分析仪中基于臭氧的化学发光对后者进行灵敏检测相结合。在此，我们报告一种新型反应，该反应易于应用于商业NO分析仪，它利用硫化氢（H2S），这种气体可将RSNO还原为NO，并且与NO类似，由内源性代谢产生且对多种生物学功能有影响。我们讨论了影响基于H2S的RSNO测量方法的因素，并探讨了H2S作为测量RSNO的实验工具的潜力。
