Walls A F, Roberts J A, Godfrey R C, Church M K, Holgate S T
Immunopharmacology Group, University of Southampton, UK.
Int Arch Allergy Appl Immunol. 1990;92(3):233-41. doi: 10.1159/000235183.
Fixation and staining characteristics were studied for mast cells recovered by bronchoalveolar lavage from 67 patients being investigated for lung disease. The number of toluidine blue stained mast cells in formaldehyde-fixed cytocentrifuge preparations was consistently less than in specimens fixed in Carnoy's solution, though the counts were highly dependent on the period of fixation or staining. The cellular histamine content closely correlated with total mast cell numbers in bronchoalveolar lavage fluid, but was not related to the relative proportions of mast cells which were sensitive or resistant to formaldehyde fixation when using a standard protocol. Compared with normal subjects, the numbers of formaldehyde-sensitive mast cells were significantly elevated in patients with bronchial carcinoma, sarcoidosis, extrinsic allergic alveolitis, cryptogenic fibrosing alveolitis, and mycobacterial infection and were particularly high in the cases of interstitial lung disease. An even greater increase in numbers of formaldehyde-resistant mast cells was observed in the patients with sarcoidosis and extrinsic allergic alveolitis. The associations of these mast cell subsets with disease may reflect relationships between the expansion of the formaldehyde-sensitive population and lymphocyte infiltration and between proliferation of formaldehyde-resistant mast cells and tissue fibrosis.
对67名因肺部疾病接受检查的患者经支气管肺泡灌洗回收的肥大细胞的固定和染色特性进行了研究。在甲醛固定的细胞离心涂片制备物中，甲苯胺蓝染色的肥大细胞数量始终少于用卡诺氏液固定的标本，尽管计数高度依赖于固定或染色时间。细胞组胺含量与支气管肺泡灌洗液中的肥大细胞总数密切相关，但在使用标准方案时，与对甲醛固定敏感或耐受的肥大细胞的相对比例无关。与正常受试者相比，支气管癌、结节病、外源性过敏性肺泡炎、隐源性纤维性肺泡炎和分枝杆菌感染患者中对甲醛敏感的肥大细胞数量显著升高，在间质性肺病患者中尤其高。在结节病和外源性过敏性肺泡炎患者中观察到对甲醛耐受的肥大细胞数量有更大的增加。这些肥大细胞亚群与疾病的关联可能反映了对甲醛敏感群体的扩增与淋巴细胞浸润之间以及对甲醛耐受的肥大细胞增殖与组织纤维化之间的关系。
