Zhang Yi-Bo, Ba Cai-Feng, Su Yu-Hong, Zeng Rui-Xia
Department of Immunology and Pathogenic Biology, Jinzhou Medical College, Jinzhou 121001, China.
Yi Chuan. 2006 Oct;28(10):1224-8. doi: 10.1360/yc-006-1224.
The relationship between MC4R gene polymorphism and body weight in beagle dogs was analyzed. Using gene-specific primers based on canine MC4R exonic sequences a gene fragment was PCR amplified, cloned and sequenced to identify potential polymorphisms. The relationship between a MC4R gene polymorphism detected by PCR-RFLP and canine body weight was analyzed. Three variants were found in beagle dog MC4R DNA sequence, of which two were deletions and one was a transversion which created a PshA I site that could be detected by PCR-RFLP. A statistically significant relationship between this polymorphism and body weight was found. MC4R gene could be a candidate modifier gene for canine body weight.
分析了比格犬MC4R基因多态性与体重之间的关系。基于犬MC4R外显子序列设计基因特异性引物，通过PCR扩增、克隆和测序一个基因片段，以鉴定潜在的多态性。分析了通过PCR-RFLP检测到的MC4R基因多态性与犬体重之间的关系。在比格犬MC4R DNA序列中发现了三个变体，其中两个是缺失，一个是导致产生可通过PCR-RFLP检测的PshA I位点的颠换。发现这种多态性与体重之间存在统计学上的显著关系。MC4R基因可能是犬体重的候选修饰基因。
