Gentilhomme E, Faure M, Piemont Y, Binder P, Thivolet J
Centre d'études du Bouchet, Vert le Petit, France.
J Dermatol. 1990 Sep;17(9):526-32. doi: 10.1111/j.1346-8138.1990.tb01689.x.
In the staphylococcal scalded skin syndrome, spontaneous intraepithelial cleavages are due to the exfoliative toxins A or B (ETA or ETB). Until now, these toxins have been studied either on epidermis or on organotypic skin cultures. In the present study, we compare the effects of these toxins on human keratinocyte cell cultures to those on human and mouse organotypic skin cultures. With concentrations of ETA or ETB of 1 mg/ml for 3 hours, spontaneous intraepithelial cleavages were noted in both cell and organotypic cultures. Keratinocyte cell cultures were as sensitive as organotypic skin cultures to these toxins. Since keratohyaline granules may represent a possible binding site for ETA or ETB, we tried to correlate the expression of keratohyaline granules with the appearance of intraepithelial clefts due to the toxins. However, when cultured in liquid medium, epithelia were not differentiated enough to allow the detection of the binding site of ETA-ETB.
在葡萄球菌性烫伤样皮肤综合征中，自发性上皮内裂隙是由剥脱毒素A或B（ETA或ETB）引起的。到目前为止，这些毒素要么在表皮上进行研究，要么在器官型皮肤培养物上进行研究。在本研究中，我们比较了这些毒素对人角质形成细胞培养物的影响与对人和小鼠器官型皮肤培养物的影响。当ETA或ETB浓度为1mg/ml作用3小时时，在细胞培养物和器官型培养物中均观察到自发性上皮内裂隙。角质形成细胞培养物对这些毒素的敏感性与器官型皮肤培养物相同。由于透明角质颗粒可能是ETA或ETB的一个可能结合位点，我们试图将透明角质颗粒的表达与毒素引起的上皮内裂隙的出现联系起来。然而，当在液体培养基中培养时，上皮细胞分化程度不够，无法检测到ETA-ETB的结合位点。
