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白色念珠菌酵母形态和菌丝形态纯化质膜的酶活性。

Enzymic activity of purified plasma membranes from the yeast and mycelial forms of Candida albicans.

作者信息

Marriott M S

出版信息

J Gen Microbiol. 1975 Aug;89(2):345-52. doi: 10.1099/00221287-89-2-345.

DOI:10.1099/00221287-89-2-345
PMID:170363
Abstract

Plasma membranes were isolated from the yeast and mycelial forms of Candida albicans as described previously (Marriott, 1975) and examined for the presence of several enzymes. Measurement of specific activities showed enrichment of Mg2+-dependent and Ma+/K+-stimulated Mg2+-dependent adenosine triphosphatase and mannan synthetase, in the plasma membrane fractions from both morphological forms of the organism. However, acid and alkaline phosphatase, NADH oxidase and 5'-nucleotidase showed no such specific location.

摘要

如前所述(Marriott,1975年),从白色念珠菌的酵母形态和菌丝形态中分离出质膜,并检测了几种酶的存在情况。比活性的测定表明,在该生物体两种形态的质膜组分中,Mg2+依赖型和Mg+/K+刺激型Mg2+依赖型三磷酸腺苷酶以及甘露聚糖合成酶均有富集。然而,酸性和碱性磷酸酶、NADH氧化酶和5'-核苷酸酶并未表现出这种特定的定位。

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引用本文的文献

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Morphogenesis of Candida albicans and cytoplasmic proteins associated with differences in morphology, strain, or temperature.白色念珠菌的形态发生以及与形态、菌株或温度差异相关的细胞质蛋白。
J Bacteriol. 1980 Oct;144(1):258-73. doi: 10.1128/jb.144.1.258-273.1980.
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Partial characterization of the plasma membrane ATPase from a rho0 petite strain of Saccharomyces cerevisiae.来自酿酒酵母rho0小菌落菌株的质膜ATP酶的部分特性分析
J Bioenerg Biomembr. 1980 Aug;12(3-4):249-64. doi: 10.1007/BF00744687.
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Subcellular fractionation of a hypercellulolytic mutant, Trichoderma reesei Rut-C30: localization of endoglucanase in microsomal fraction.
Appl Environ Microbiol. 1985 Nov;50(5):1137-43. doi: 10.1128/aem.50.5.1137-1143.1985.
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Dimorphism-associated changes in plasma membrane H(+)-ATPase activity of Candida albicans.白色念珠菌质膜H(+) -ATP酶活性与二态性相关的变化
Arch Microbiol. 1991;156(5):412-5. doi: 10.1007/BF00248719.