大鼠肝脏微粒体和分离的亚细胞膜的分析研究。I. 生化方法。
Analytical study of microsomes and isolated subcellular membranes from rat liver. I. Biochemical methods.
作者信息
Beaufay H, Amar-Costesec A, Feytmans E, Thinès-Sempoux D, Wibo M, Robbi M, Berthet J
出版信息
J Cell Biol. 1974 Apr;61(1):188-200. doi: 10.1083/jcb.61.1.188.
Abstract
The series introduced by this paper reports the results of a detailed analysis of the microsomal fraction from rat liver by density gradient centrifugation. The biochemical methods used throughout this work for the determination of monoamine oxidase, NADH cytochrome c reductase, NADPH cytochrome c reductase, cytochrome oxidase, catalase, aminopyrine demethylase, cytochromes b(5) and P 450, glucuronyltransferase, galactosyltransferase, esterase, alkaline and acid phosphatases, 5'-nucleotidase, glucose 6-phosphatase, alkaline phosphodiesterase I, N-acetyl-beta-glucosaminidase, beta-glucuronidase, nucleoside diphosphatase, aldolase, fumarase, glutamine synthetase, protein, phospholipid, cholesterol, and RNA are described and justified when necessary.
摘要
本文介绍的系列报告了通过密度梯度离心对大鼠肝脏微粒体部分进行详细分析的结果。本文通篇使用的用于测定单胺氧化酶、NADH细胞色素c还原酶、NADPH细胞色素c还原酶、细胞色素氧化酶、过氧化氢酶、氨基比林脱甲基酶、细胞色素b(5)和P450、葡糖醛酸基转移酶、半乳糖基转移酶、酯酶、碱性和酸性磷酸酶、5'-核苷酸酶、葡萄糖6-磷酸酶、碱性磷酸二酯酶I、N-乙酰-β-葡糖胺酶、β-葡糖醛酸酶、核苷二磷酸酶、醛缩酶、延胡索酸酶、谷氨酰胺合成酶、蛋白质、磷脂、胆固醇和RNA的生化方法在必要时进行了描述和论证。
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Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmic granules in rat liver.组织分级分离研究。5. 大鼠肝脏中酸性磷酸酶与一类特殊细胞质颗粒的关联。
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