Tang Shusheng, Li Defa, Qiao Shiyan, Piao Xiangshu, Zang Jianjun
National Key Lab of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing.
Arch Anim Nutr. 2006 Oct;60(5):418-26. doi: 10.1080/17450390600884450.
The objective of this study was to investigate the effect of purified soybean agglutinin on growth and immune function in rats. Thirty male Sprague-Dawley rats (77.8 +/- 2.6 g) were individually fed casein-cornstarch based diets containing 0, 0.05, 0.10, 0.15 or 0.20% soybean agglutinin (w/w) during a 20-day experiment. Growth declined linearly with increasing the concentration of soybean agglutinin (p < 0.05). The proliferation of lymphocytes in spleen, lymph nodes and blood decreased with an increase in dietary soybean agglutinin (p < 0.05). The concentrations of interleukin-2, interferon-gamma and tumor necrosis factor-alpha in plasma, spleen, and mesenteric lymph nodes as well as plasma concentrations of IgA, IgG and IgM also declined with increasing dose of soybean agglutinin (p < 0.05). The results show that dietary soybean agglutinin has negative effects on growth as well as both cell-mediated and humoral immune function of rats and appears to function in a dose-dependent manner.
本研究的目的是探讨纯化大豆凝集素对大鼠生长和免疫功能的影响。在为期20天的实验中，将30只雄性斯普拉格-道利大鼠（77.8±2.6克）分别喂食含0、0.05、0.10、0.15或0.20%大豆凝集素（w/w）的酪蛋白-玉米淀粉基日粮。随着大豆凝集素浓度的增加，生长呈线性下降（p<0.05）。随着日粮中大豆凝集素含量的增加，脾脏、淋巴结和血液中淋巴细胞的增殖减少（p<0.05）。血浆、脾脏和肠系膜淋巴结中白细胞介素-2、干扰素-γ和肿瘤坏死因子-α的浓度以及血浆中IgA、IgG和IgM的浓度也随着大豆凝集素剂量的增加而下降（p<0.05）。结果表明，日粮中的大豆凝集素对大鼠的生长以及细胞介导免疫和体液免疫功能均有负面影响，且似乎呈剂量依赖性。
