Zhu Yuanzhao, Li Defa, Wang Fenglai, Yin Jingdong, Jin Hong
National Feed Engineering Technology Research Center, China Agricultural University, Beijing, China.
Arch Anim Nutr. 2004 Aug;58(4):295-310. doi: 10.1080/00039420412331273277.
This study was conducted to compare the safety of soybean meal prepared from genetically modified (GM) glyphosate-tolerant (Roundup Ready; RR) soybeans and conventional soybeans. Eighty Sprague-Dawley rats (40 males and 40 females) were randomly allotted to one of four groups according to sex and body weight for a 13-week feeding experiment. The rats were fed corn-based diets containing 60% conventional soybean meal, a mixture of 30% conventional and 30% RR soybean meal, 60% or 90% RR soybean meal. All diets were adjusted to an identical nutrient level except the 90% RR diet. The two soybean meals were similar in chemical analysis and amino acid composition. During the 13-week growth trial, body weight (P < 0.05) and feed intake (P < 0.05) decreased only in rats fed with 90% RR soybean meal at the first week. No treatment-related deaths occurred during the experiment. Gross necropsy findings, haematological or urinalysis values and clinical serum parameters showed no meaningful differences between rats fed the control and RR soybean meals. A 145 bp of cp4 epsps gene specific for the GM constructs from RR soybean meal or a 407 bp of lec gene from endogenous soybean DNA could not be detected in investigated masseter muscle samples. No adverse effects of glyphosate-tolerant soybean meal on rats were seen even at levels as high as 90% of the diet.
本研究旨在比较由转基因抗草甘膦（农达抗性；RR）大豆制备的豆粕与传统大豆豆粕的安全性。80只斯普拉格-道利大鼠（40只雄性和40只雌性）根据性别和体重被随机分配到四组中的一组，进行为期13周的喂养实验。大鼠被喂食以玉米为基础的日粮，其中含有60%的传统豆粕、30%传统豆粕与30%RR豆粕的混合物、60%或90%的RR豆粕。除了90%RR日粮外，所有日粮均调整至相同的营养水平。两种豆粕在化学分析和氨基酸组成方面相似。在13周的生长试验中，仅在第一周喂食90%RR豆粕的大鼠中体重（P<0.05）和采食量（P<0.05）有所下降。实验期间未发生与处理相关的死亡。大体尸检结果、血液学或尿液分析值以及临床血清参数在喂食对照豆粕和RR豆粕的大鼠之间未显示出有意义的差异。在所研究的咬肌样本中未检测到来自RR豆粕的GM构建体特异的145bp的cp4 epsps基因或来自内源性大豆DNA的407bp的lec基因。即使日粮中RR豆粕含量高达90%，也未观察到其对大鼠有不良影响。
