Lehtola M J, Pitkänen T, Miebach L, Miettinen I T
Laboratory of Environmental Microbiology, National Public Health Institute, P.O. Box 95, FI-70701 Kuopio, Finland.
Water Sci Technol. 2006;54(3):57-61. doi: 10.2166/wst.2006.448.
Campylobacteria are important foodborne pathogens. C. jejuni bacteria have caused several drinking water-related epidemics in Finland. Normally, C. jejuni is not able to multiply in drinking water or in biofilms although it may survive in biofilms. The survival of C. jejuni in biofilms was studied using the Propella biofilm reactor. The number of bacteria was analysed with traditional culture methods and with fluorescence in situ hybridisation (FISH). By culture methods C. jejuni was detectable for only 1 d after spiking whereas bacteria were found from biofilms for at least 1 week after spiking and from outlet water of the reactor for 3 weeks when using FISH. These results suggested that C. jejuni may survive in biofilms and culture methods probably seriously underestimate the real number in water and in biofilms.
弯曲杆菌是重要的食源性病原体。空肠弯曲杆菌在芬兰已引发了几起与饮用水相关的疫情。通常情况下，空肠弯曲杆菌虽能在生物膜中存活，但无法在饮用水或生物膜中繁殖。利用Propella生物膜反应器研究了空肠弯曲杆菌在生物膜中的存活情况。采用传统培养方法和荧光原位杂交（FISH）技术分析细菌数量。通过培养方法，接种后仅1天可检测到空肠弯曲杆菌，而使用FISH技术时，接种后至少1周可在生物膜中发现细菌，在反应器出水口中3周仍可发现细菌。这些结果表明，空肠弯曲杆菌可能在生物膜中存活，且培养方法可能严重低估了水中和生物膜中的实际细菌数量。
