Monogastric Science Research Center, Scotland Rural College (SRUC), Ayr, KA6 5HW, Scotland, UK.
School of Chemistry, Center for Biomolecular Sciences, University of Nottingham, NG7 2RD, UK.
Poult Sci. 2018 Apr 1;97(4):1391-1399. doi: 10.3382/ps/pex413.
Reducing Campylobacter spp. carriage in poultry is challenging, but essential to control this major cause of human bacterial gastroenteritis worldwide. Although much is known about the mechanisms and route of Campylobacter spp. colonization in poultry, the literature is scarce on antibiotic-free solutions to combat Campylobacter spp. colonization in poultry. In vitro and in vivo studies were conducted to investigate the role of TYPLEX® Chelate (ferric tyrosine), a novel feed additive, in inhibiting Campylobacter jejuni (C. jejuni) biofilm formation and reducing C. jejuni and Escherichia coli (E. coli) colonization in broiler chickens at market age. In an in vitro study, the inhibitory effect on C. jejuni biofilm formation using a plastic bead assay was investigated. The results demonstrated that TYPLEX® Chelate significantly reduces biofilm formation. In an in vivo study, 800 broilers (one d old) were randomly allocated to 4 dietary treatments in a randomized block design, each having 10 replicate pens with 20 birds per pen. At d 21, all birds were challenged with C. jejuni via seeded litter. At d 42, cecal samples were collected and tested for volatile fatty acid (VFA) concentrations and C. jejuni and E. coli counts. The results showed that TYPLEX® Chelate reduced the carriage of C. jejuni and E. coli in poultry by 2 and 1 log10 per gram cecal sample, respectively, and increased cecal VFA concentrations. These findings support TYPLEX® Chelate as a novel non-antibiotic feed additive that may help produce poultry with a lower public health risk of Campylobacteriosis.
减少家禽中弯曲杆菌属的携带量具有挑战性，但对于控制这种全球范围内人类细菌性肠胃炎的主要原因至关重要。尽管人们对弯曲杆菌属在禽类中的定植机制和途径有了很多了解，但关于在禽类中对抗弯曲杆菌属定植的无抗生素解决方案的文献却很少。进行了体外和体内研究，以研究 TYPLEX®Chelate（高铁酪氨酸）作为一种新型饲料添加剂，在抑制空肠弯曲杆菌（C. jejuni）生物膜形成和减少肉鸡上市时空肠弯曲杆菌和大肠杆菌（E. coli）定植中的作用。在一项体外研究中，使用塑料珠测定法研究了 TYPLEX®Chelate 对空肠弯曲杆菌生物膜形成的抑制作用。结果表明，TYPLEX®Chelate 可显著减少生物膜形成。在一项体内研究中，将 800 只肉鸡（1 日龄）随机分配到 4 种饮食处理中，采用随机分组设计，每个处理有 10 个重复栏，每个栏有 20 只鸡。在第 21 天，所有的鸡都通过接种的垫料感染空肠弯曲杆菌。在第 42 天，收集盲肠样本并测试挥发性脂肪酸（VFA）浓度以及空肠弯曲杆菌和大肠杆菌的数量。结果表明，TYPLEX®Chelate 可使家禽中携带的空肠弯曲杆菌和大肠杆菌分别减少 2 和 1 个对数 10 个/克盲肠样本，并且增加盲肠 VFA 浓度。这些发现支持 TYPLEX®Chelate 作为一种新型的非抗生素饲料添加剂，有助于生产出公共卫生风险较低的弯曲杆菌病家禽。
