Alonso M C, Dionisio L P C, Bosch A, de Moura B S Pereira, Garcia-Rosado E, Borrego J J
Dept of Microbiology, Campus Universitario Teatinos, University of Malaga, 29071-Malaga, Spain.
Water Sci Technol. 2006;54(3):109-17. doi: 10.2166/wst.2006.456.
Microbial quality of reclaimed water used for irrigation in two golf courses located in the southern Iberian Peninsula (Spain and Portugal) was evaluated. Bacterial indicators for faecal pollution (total and faecal coliforms, Escherichia coli and enterococci) were tested by membrane filtration using appropriate selective media. In addition, somatic E. coli bacteriophages, enteric viruses (entero-, hepatitis A and rota-) and Legionella pneumophila were also analysed. The results obtained showed that all wastewater treatment processes reduced adequately the number of indicator microorganisms although a significant correlation between pathogenic and indicator microorganisms tested was not found. L. pneumophila was detected by PCR but not confirmed by culture. Survival experiments of pathogenic microorganisms in aerosols and irrigated turf are conducted to determine the health hazards for the golf practice and to propose a microbial standard for wastewater used for irrigation of golf courses.
对位于伊比利亚半岛南部（西班牙和葡萄牙）的两个高尔夫球场用于灌溉的再生水的微生物质量进行了评估。使用适当的选择性培养基通过膜过滤法检测粪便污染的细菌指标（总大肠菌群和粪大肠菌群、大肠杆菌和肠球菌）。此外，还分析了体细胞大肠杆菌噬菌体、肠道病毒（肠道病毒、甲型肝炎病毒和轮状病毒）和嗜肺军团菌。所得结果表明，尽管未发现所检测的致病微生物与指示微生物之间存在显著相关性，但所有废水处理工艺都充分减少了指示微生物的数量。通过聚合酶链反应检测到了嗜肺军团菌，但培养未得到证实。进行了致病微生物在气溶胶和灌溉草坪中的存活实验，以确定高尔夫运动对健康的危害，并为高尔夫球场灌溉用水提出微生物标准。
