Lucena F, Ribas F, Duran A E, Skraber S, Gantzer C, Campos C, Morón A, Calderón E, Jofre J
Department of Microbiology, University of Barcelona, Barcelona, Spain.
J Appl Microbiol. 2006 Jul;101(1):96-102. doi: 10.1111/j.1365-2672.2006.02907.x.
The aim of this research was to determine the suitability of coliphages (bacteriophages) for assessing the microbial quality of groundwater.
The number of several bacterial indicators (faecal coliforms, Escherichia coli, enterococci and spores of sulfite-reducing clostridia) and bacteriophages (somatic coliphages, F-specific RNA bacteriophages and bacteriophages infecting Bacteroides fragilis) were determined in groundwater of aquifers in various geographical areas. Results show that the relative abundance, determined as percentages of positive detections, of the bacterial indicators and bacteriophages varies depending on the aquifer.
A single bacterial indicator may not be enough to assess microbiological quality in certain aquifers. One bacterial indicator and a bacteriophage parameter provide more information than two bacterial indicators.
Coliphages (CPH) provide different information from that provided by bacterial indicators on the microbial quality of groundwater in different geographical areas. Easy, fast and inexpensive methods for the detection of CPH are feasible in both industrialized and developing countries.
本研究的目的是确定大肠杆菌噬菌体（噬菌体）用于评估地下水微生物质量的适用性。
测定了不同地理区域含水层地下水中几种细菌指标（粪大肠菌群、大肠杆菌、肠球菌和亚硫酸盐还原梭菌芽孢）和噬菌体（体细胞大肠杆菌噬菌体、F特异性RNA噬菌体和感染脆弱拟杆菌的噬菌体）的数量。结果表明，细菌指标和噬菌体的相对丰度（以阳性检测的百分比确定）因含水层而异。
单一细菌指标可能不足以评估某些含水层的微生物质量。一个细菌指标和一个噬菌体参数比两个细菌指标提供更多信息。
大肠杆菌噬菌体（CPH）在不同地理区域的地下水中微生物质量方面提供了与细菌指标不同的信息。在工业化国家和发展中国家，检测CPH的简便、快速且廉价的方法都是可行的。
