Anderson M E, Sobsey M D
University of North Carolina at Chapel Hill, School of Public Health, Rosenau Hall CB#7400, Chapel Hill, NC 27599-7400, USA.
Water Sci Technol. 2006;54(3):211-8. doi: 10.2166/wst.2006.471.
The use of antibiotics for growth promotion and disease treatment by the commercial swine industry has led to high proportions of multiple antibiotic-resistant enteric bacteria being shed by these animals and concerns about the environmental spread of these bacteria. A study was conducted to quantify the extent of release of antibiotic-resistant E. coli from swine farms into groundwater. Four study sites, two swine farms and two reference sites (crop farms), with known groundwater flow paths were screened for E. coli four times over the course of one and a half years. A total of 100 biochemically-confirmed E. coli were collected from the four sites. There were statistically significantly higher E. coli levels at the two swine farm sites than at the reference sites. The bacterial isolates were tested for antibiotic resistance using a panel of 17 drugs that are typical of human and veterinary use. There were 19 and 71 E. coli isolates from swine farms #1 and #2, respectively, with most (68%) being resistant to 1 -6 antimicrobials. Only one E. coli isolate from each of the reference sites showed antimicrobial resistance traits. The results of this study demonstrate that antibiotic-resistant E. coli strains are present in groundwaters of swine farms with a typical lagoon and land application system for waste management.
商业养猪业将抗生素用于促进生长和疾病治疗，导致这些动物排出高比例的多重耐药肠道细菌，并引发了对这些细菌在环境中传播的担忧。开展了一项研究，以量化养猪场中耐抗生素大肠杆菌向地下水释放的程度。在一年半的时间里，对四个研究地点（两个养猪场和两个参考地点[农作物农场]）进行了四次大肠杆菌筛查，这些地点的地下水流路径已知。从这四个地点总共收集了100株经生化确认的大肠杆菌。两个养猪场地点的大肠杆菌水平在统计学上显著高于参考地点。使用一组17种典型的人用和兽用药物对细菌分离株进行了抗生素耐药性测试。养猪场1号和2号分别有19株和71株大肠杆菌分离株，其中大多数（68%）对1至6种抗菌药物耐药。每个参考地点仅一株大肠杆菌分离株显示出抗菌耐药特性。这项研究的结果表明，在采用典型泻湖和土地施用系统进行废物管理的养猪场地下水中存在耐抗生素大肠杆菌菌株。
