Aggad H, Boukraa L
Department of Veterinary Sciences, Faculty of Agronomic and Veterinary Sciences, Tiaret University, Tiaret, Algeria.
East Mediterr Health J. 2006 Jan-Mar;12(1-2):119-28.
A serological study was carried out in Tiaret province in western Algeria on 1032 cows distributed in 95 flocks to estimate the prevalence of Brucella infection and to compare the sensitivity and specificity of a range of agglutination tests. Screening tests showed 31.5% of herds positive using the buffered plate antigen test and 26.3% using the rose Bengal test compared with 15.7% with the complement fixation test. Using the complement fixation test as the gold standard for confirmatory tests, the Rivanol test was found to be more sensitive but less specific than tube agglutination in detecting brucellosis infection. Three isolates were identified from 105 blood samples from humans with brucellosis and 50 samples of milk and tissues from infected cows and they were all Brucella melitensis biovar 3.
在阿尔及利亚西部的提亚雷特省,对分布于95个畜群中的1032头奶牛进行了一项血清学研究,以估计布鲁氏菌感染的流行率,并比较一系列凝集试验的敏感性和特异性。筛查试验显示,使用缓冲板抗原试验时,31.5%的畜群呈阳性;使用玫瑰红试验时,26.3%的畜群呈阳性;而补体结合试验的阳性率为15.7%。以补体结合试验作为确诊试验的金标准,发现利凡诺试验在检测布鲁氏菌病感染方面比试管凝集试验更敏感,但特异性更低。从105份布病患者血液样本以及50份感染奶牛的牛奶和组织样本中鉴定出3株菌株,它们均为布鲁氏菌羊种生物变种3。