Adabi M, Gharekhani J, Alamian S, Varasteh Shams M, Fathi Sheikh M, Shivapour Z, Valiei Z, Khazaiee S, Ghaderi H
Infectious Diseases Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Infectious Ophthalmologic Research Center, Imam Khomeini Hospital Clinical Research Development Unit, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Iran J Vet Res. 2024;25(2):156-160. doi: 10.22099/IJVR.2024.49557.7292.
Timely diagnosis of brucellosis is the starting point for effective programs to control brucellosis in humans and animals. This study aimed to detect infection in milk samples from livestock in Famenin, an endemic region of western Iran, using a milk ring test and molecular techniques.
In this cross-sectional study, 738 raw milk samples were randomly collected from cattle, sheep, and goats. Milk samples were screened using the milk ring test (MRT). In addition, polymerase chain reaction (PCR) method was applied to detect spp. in all MRT-positive samples. DNA from the milk samples was extracted and used for PCR using the and loci.
Of the samples tested using MRT, 46 (6.23%, 95% CI: 2.83-9.63%) yielded positive results. Of the 46 seropositive samples, 42 (91.30%) were from sheep and 4 (8.70%) were from goats, while no bovine samples had positive MRT results. PCR analysis confirmed that 78.26% (36/46) of MRT-positive samples belonged to the genus . Furthermore, 83.33% (30/36) of the confirmed samples were identified as , while 16.66% (6/36) were identified as .
The results obtained from MRT evaluation of milk samples did not align entirely with the findings of the molecular examinations. The PCR method has minimal biological contamination and high sensitivity and accuracy, especially for determining species. Raw milk should be routinely assessed for contamination. This work is necessary to identify hidden infections and break the chain of transmission of brucellosis.
布鲁氏菌病的及时诊断是控制人类和动物布鲁氏菌病有效计划的起点。本研究旨在使用乳环试验和分子技术检测伊朗西部布鲁氏菌病流行区法梅宁家畜乳样中的感染情况。
在这项横断面研究中，从牛、绵羊和山羊中随机采集了738份生鲜乳样。使用乳环试验（MRT）对乳样进行筛查。此外，应用聚合酶链反应（PCR）方法检测所有MRT阳性样品中的布鲁氏菌属。从乳样中提取DNA，并使用bcsp31和omp25基因座进行PCR。
在使用MRT检测的样品中，46份（6.23%，95%可信区间：2.83 - 9.63%）结果呈阳性。在46份血清学阳性样品中，42份（91.30%）来自绵羊，4份（8.70%）来自山羊，而没有牛样的MRT结果呈阳性。PCR分析证实，78.26%（36/46）的MRT阳性样品属于布鲁氏菌属。此外，83.33%（30/36）的确诊样品被鉴定为流产布鲁氏菌，而16.66%（6/36）被鉴定为马尔他布鲁氏菌。
乳样MRT评估结果与分子检测结果并不完全一致。PCR方法生物污染极小，灵敏度和准确性高，尤其对于确定布鲁氏菌物种。应常规评估生鲜乳中布鲁氏菌的污染情况。这项工作对于识别隐性感染和打破布鲁氏菌病传播链是必要的。
