Calvino B, Couraud J Y, Maillet S, Pradelles P, Besson J M
INSERM U161, Laboratoire de Recherches de Neurophysiologie Pharmacologique, Paris, France.
Brain Res. 1988 Sep 20;460(2):389-93. doi: 10.1016/0006-8993(88)90388-5.
Mice immunized against anti-substance P (anti-SP) monoclonal antibodies produced anti-SP anti-idiotypic antibodies (SPAb2). In a previous report. SPAb2 antibodies were found to have in vitro biological activity i.e. to behave either as agonists or as antagonists for substance P (SP) depending on the biological test. In this study, the involvement of SPAb2 in in vivo biological activity has been tested. Because of the possible implication of SP in the generation and transmission of nociceptive information, we have tested the responsiveness of SPAb2 responding mice in behavioral nociceptive tests. SPAb2 mice showed very small behavioral variations in the hot plate test as compared with a control group of mice immunized against an unrelated monoclonal antibody. In the formalin test, however, SPAb2 mice displayed a significant increase in paw licking time, which was significantly correlated with SPAb2 serum concentration. These results are discussed in terms of the use of SPAb2 as pharmacological tools for studying the biological properties of SP receptors and more generally of auto anti-idiotypic antibodies in modulating behavioral responses.
用抗P物质（抗SP）单克隆抗体免疫的小鼠产生了抗SP抗独特型抗体（SPAb2）。在之前的一份报告中，发现SPAb2抗体具有体外生物学活性，即根据生物学试验，其表现为P物质（SP）的激动剂或拮抗剂。在本研究中，已测试了SPAb2在体内生物学活性中的作用。由于SP可能参与伤害性信息的产生和传递，我们在行为伤害性试验中测试了对SPAb2有反应的小鼠的反应性。与用无关单克隆抗体免疫的对照组小鼠相比，SPAb2小鼠在热板试验中的行为变化非常小。然而，在福尔马林试验中，SPAb2小鼠的舔爪时间显著增加，这与SPAb2血清浓度显著相关。就使用SPAb2作为研究SP受体生物学特性以及更普遍地研究自身抗独特型抗体调节行为反应的药理学工具而言，对这些结果进行了讨论。
