Hadj-Kaddour K, Carcy B, Vallet A, Randazzo S, Delbecq S, Kleuskens J, Schetters T, Gorenflot A, Precigout E
Laboratoire Biologie Cellulaire and Moléculaire, ERT 1038 'Vaccination anti-parasitaire', UFR Pharmacie, Université Montpellier I, 15 Avenue Charles Flahault, B.P. 14491, 34093 Montpellier Cedex 5, France.
Parasitology. 2007 Feb;134(Pt 2):187-96. doi: 10.1017/S0031182006001399. Epub 2006 Oct 13.
The Bd37gene encoding for a glycosyl-phosphatidyl-inositol anchored protein of Babesia divergens displays genetic polymorphisms among isolates. Five major polymorphic groups (clades) were shown by PCR-RFLP among different B. divergens isolates. Each group has been characterized according to a reference Bd37 gene (Rouen87, W8843, Y5, 6303E and 1705B). Recombinant (GST fusion) protein (Bd37r) expressed from the Bd37 gene, was used as antigen in a saponin-based formulation and was able to protect gerbils, after 2 injections at low dose vaccine concentration (1 mug per dose), against a virulent challenge with the B. divergens Rouen87 isolate. In spite of polymorphism of Bd37 gene, Bd37r induced complete immunoprotection against challenges with each of the 5 reference isolate groups defined by PCR-RFLP.
编码分歧巴贝斯虫糖基磷脂酰肌醇锚定蛋白的Bd37基因在分离株中表现出遗传多态性。通过PCR-RFLP分析，不同的分歧巴贝斯虫分离株显示出五个主要的多态性组（进化枝）。每个组已根据参考Bd37基因（鲁昂87、W8843、Y5、6303E和1705B）进行了表征。从Bd37基因表达的重组（GST融合）蛋白（Bd37r），在低剂量疫苗浓度（每剂1微克）下注射2次后，被用作基于皂苷配方的抗原，能够保护沙鼠免受分歧巴贝斯虫鲁昂87分离株的强毒攻击。尽管Bd37基因存在多态性，但Bd37r诱导了针对由PCR-RFLP定义的5个参考分离株组中每一组攻击的完全免疫保护。
