Urven Lance E, Yabe Taijiro, Pelegri Francisco
Laboratory of Genetics, University of Wisconsin - Madison, WI 53706, USA.
J Cell Sci. 2006 Oct 15;119(Pt 20):4342-52. doi: 10.1242/jcs.03197.
Cytokinesis in early zebrafish embryos involves coordinated changes in the f-actin- and microtubule-based cytoskeleton, and the recruitment of adhesion junction components to the furrow. We show that exposure to inhibitors of non-muscle myosin II function does not affect furrow ingression during the early cleavage cycles but interferes with the recruitment of pericleavage f-actin and cortical beta-catenin aggregates to the furrow, as well as the remodeling of the furrow microtubule array. This remodeling is in turn required for the distal aggregation of the zebrafish germ plasm. Embryos with reduced myosin activity also exhibit at late stages of cytokinesis a stabilized contractile ring apparatus that appears as a ladder-like pattern of short f-actin cables, supporting a role for myosin function in the disassembly of the contractile ring after furrow formation. Our studies support a role for myosin function in furrow maturation that is independent of furrow ingression and which is essential for the recruitment of furrow components and the remodeling of the cytoskeleton during cytokinesis.
斑马鱼早期胚胎中的胞质分裂涉及基于F-肌动蛋白和微管的细胞骨架的协调变化，以及粘附连接成分向沟的募集。我们发现，暴露于非肌肉肌球蛋白II功能抑制剂不会影响早期卵裂周期中的沟内陷，但会干扰卵裂期F-肌动蛋白和皮质β-连环蛋白聚集体向沟的募集，以及沟微管阵列的重塑。这种重塑反过来又是斑马鱼生殖质远端聚集所必需的。肌球蛋白活性降低的胚胎在胞质分裂后期还表现出稳定的收缩环装置，呈现为短F-肌动蛋白电缆的梯状模式，支持肌球蛋白功能在沟形成后收缩环解体中的作用。我们的研究支持肌球蛋白功能在沟成熟中的作用，该作用独立于沟内陷，并且对于胞质分裂期间沟成分的募集和细胞骨架的重塑至关重要。
