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[抗HIV-1/2联合酶联免疫吸附测定的初步结果]

[Initial results of the anti-HIV-1/2 combined ELISA].

作者信息

Bäcker U, Gathof B, Eberle J, Gürtler L

机构信息

Blutspendedienst des Bayerischen Roten Kreuzes, München.

出版信息

Beitr Infusionsther. 1990;26:9-11.

PMID:1703910
Abstract

Between March 30 and July 31, 1989, 117,874 blood donations were tested for anti-HIV-1 and in a combined ELISA for anti-HIV-1/HIV-2 (both Abbott). On each repeat reactive (14 = 0.01% for anti-HIV-1 and 400 = 0.34% for anti-HIV-1/HIV-2), a Western blot (WB) was performed. Two donors, who tested repeatedly reactive in both ELISAs, were confirmed anti-HIV-1 positive by WB. As the specificity of anti-HIV-1/HIV-2 ELISA is considerably lower (99.66%) than of anti-HIV-1 (99.99%), introduction of a different HIV-2 specific ELISA should be discussed to prevent loss of too many blood donations and donors through anti-HIV screening.

摘要

1989年3月30日至7月31日期间,对117,874份献血样本进行了抗HIV-1检测,并采用联合酶联免疫吸附测定法检测抗HIV-1/HIV-2(均为雅培试剂)。每次重复检测呈反应性时(抗HIV-1检测中14份呈反应性,占0.01%;抗HIV-1/HIV-2检测中400份呈反应性,占0.34%),均进行蛋白印迹法(WB)检测。两名在两种酶联免疫吸附测定法中均重复检测呈反应性的献血者,经蛋白印迹法确认为抗HIV-1阳性。由于抗HIV-1/HIV-2酶联免疫吸附测定法的特异性(99.66%)远低于抗HIV-1(99.99%),因此应讨论引入不同的HIV-2特异性酶联免疫吸附测定法,以防止因抗HIV筛查导致过多献血样本和献血者流失。

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