Becker J, Becker J L, Best-Belpomme M
Université Pierre et Marie Curie, U.A. CNRS 1135, Paris, France.
Cell Mol Biol. 1990;36(4):449-60.
DNA-RNA complexes were characterized and purified in a Drosophila melanogaster cell line. Such duplexes were shown to be specific of 1731 and other "copia-like" transposable elements. DNA-RNA complexes were purified through a Sephadex G-75 column from a global nucleic acid preparation or from a total RNA fraction prior to DNA-A and RNA-A treatment. They incorporated both labelled thymidine and uridine and their resistance or sensibility to enzymes or chemicals was consistent with that being expected with such hybrid molecules. From that intermediate form of reverse transcription, the resulting labelled cDNAs were obtained and were shown to be homologous to different drosophila "copia-like" transposable elements. These results suggest that most of the "copia-like" transposable elements were amplified through a reverse transcription pathway in Drosophila melanogaster.
在果蝇细胞系中对DNA-RNA复合物进行了表征和纯化。这类双链体被证明是1731及其他“类柯皮亚”转座元件所特有的。DNA-RNA复合物通过Sephadex G-75柱从总体核酸制剂或在DNA-A和RNA-A处理之前从总RNA组分中进行纯化。它们既掺入了标记的胸苷也掺入了尿苷，并且它们对酶或化学物质的抗性或敏感性与这类杂交分子所预期的一致。从逆转录的这种中间形式获得了产生的标记cDNA，并显示其与不同的果蝇“类柯皮亚”转座元件同源。这些结果表明，大多数“类柯皮亚”转座元件是通过果蝇中的逆转录途径进行扩增的。
