Hanafy Ahmed, Ito Junji, Iida Soji, Kang Yingqian, Kogure Takahisa, Yazawa Katsukiyo, Yaguchi Takashi, Mikami Yuzuru
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8673, Japan.
Mycopathologia. 2006 Oct;162(4):281-7. doi: 10.1007/s11046-006-0045-6.
In Japan during 1996-2004, 21 actinomycete strains that have madurose as the diagnostic cell-wall sugar and show true branching in their substrate and aerial mycelia were isolated from sputa or bronchoalveolar lavage (BAL) fluid of patients with pulmonary infections or who were suspected of having related infections. Chemotaxonomic studies showed that all the isolates belong to the genus Actinomadura. Among them, six and seven strains were classified respectively into clusters of Actinomadura nitritigenes and Actinomadura cremea based on 16S rDNA analyses because their 16S rDNA similarities to those respective species were greater than 99.5%. To our knowledge, this is first report that strains of above two species were isolated from clinical specimens. Neither Actinomadura madurae nor Actinomadura pelletieri strain was isolated, and one new species, Actinomadura chibensis, was proposed; the remaining seven strains were not assigned into any known species, suggesting the presence of another new Actinomadura species.
1996年至2004年期间，在日本，从肺部感染患者或疑似患有相关感染患者的痰液或支气管肺泡灌洗（BAL）液中分离出21株放线菌菌株，这些菌株以马杜拉糖作为诊断性细胞壁糖，在其基内菌丝和气生菌丝中呈现真正的分支。化学分类学研究表明，所有分离株均属于马杜拉放线菌属。其中，基于16S rDNA分析，分别有6株和7株菌株被归类到硝化马杜拉放线菌和乳黄马杜拉放线菌聚类中，因为它们与相应物种的16S rDNA相似性大于99.5%。据我们所知，这是首次报道从临床标本中分离出上述两个物种的菌株。未分离到马杜拉马杜拉放线菌和佩氏马杜拉放线菌菌株，并提出了一个新物种——知本马杜拉放线菌；其余7株菌株未被归入任何已知物种，这表明存在另一种新的马杜拉放线菌物种。
