Kageyama Akiko, Yazawa Katsukiyo, Nishimura Kazuko, Mikami Yuzuru
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan.
Int J Syst Evol Microbiol. 2004 Mar;54(Pt 2):563-569. doi: 10.1099/ijs.0.02794-0.
Comparative 16S rDNA studies on six strains of actinomycete isolated from clinical specimens revealed that they belong to the genus Nocardia and are closely related to Nocardia seriolae, Nocardia otitidiscaviarum, Nocardia uniformis, Nocardia pseudobrasiliensis and Nocardia crassostreae. However, the novel organisms consistently formed a clade distinct from that of the five latter species. Determination of DNA-DNA relatedness indicated that these strains could be classified under three novel species. Based on their phenotypic and phylogenetic characters, three novel species of the genus Nocardia are established: Nocardia inohanensis sp. nov. for IFM 0092(T) (=NBRC 100128(T)=JCM 11891(T)=DSM 44667(T)), Nocardia yamanashiensis sp. nov. for IFM 0265(T) (=NBRC 100130(T)=JCM 11893(T)=DSM 44669(T)) and Nocardia niigatensis sp. nov. for IFM 0330(T) (=NBRC 100131(T)=JCM 11894(T)=DSM 44670(T)), IFM 0260, IFM 0636 and IFM 0833.
对从临床标本中分离出的6株放线菌进行的16S rDNA比较研究表明,它们属于诺卡氏菌属,与鰤诺卡氏菌、耳炎诺卡氏菌、均匀诺卡氏菌、伪巴西诺卡氏菌和厚壳贻贝诺卡氏菌密切相关。然而,这些新菌株始终形成一个与后五个物种不同的进化枝。DNA-DNA相关性测定表明,这些菌株可归为三个新物种。根据它们的表型和系统发育特征,建立了诺卡氏菌属的三个新物种:以IFM 0092(T)(=NBRC 100128(T)=JCM 11891(T)=DSM 44667(T))为模式菌株的稻场诺卡氏菌新种,以IFM 0265(T)(=NBRC 100130(T)=JCM 11893(T)=DSM 44669(T))为模式菌株的山梨诺卡氏菌新种,以及以IFM 0330(T)(=NBRC 100131(T)=JCM 11894(T)=DSM 44670(T))、IFM 0260、IFM 0636和IFM 0833为模式菌株的松本诺卡氏菌新种。