Movérare R, Kosunen T U, Haahtela T
Phadia AB and Uppsala University, Department of Medical Sciences, Respiratory Medicine and Allergology, Uppsala, Sweden.
J Investig Allergol Clin Immunol. 2006;16(5):274-8.
Several studies have shown that the prevalence of allergy and allergen sensitization has increased in recent years. However, the changes in the pattern of IgE reactivity to individual allergens are mostly unknown.
The aim of this preliminary study was to assess the change in IgE reactivity profile to individual timothy grass and/or birch pollen allergens in sera from sensitized individuals randomly collected 20 years apart.
Serum samples from 51 sensitized individuals were obtained from 2 cross-sectional surveys performed in 1973 and 1994 using random samples from Vammala, Finland. The sera were analyzed for IgE reactivity to timothy grass and/or birch pollen extracts, recombinant (r)Phl p 1, 2, 5, 6, 7, 11, 12, native (n)Phl p 4, and rBet v 1, 2 and 4 by immunoassay (ImmunoCAP).
The median (range) concentrations of IgE antibodies to timothy grass and birch pollen were higher in 1994 than in 1973 (6.47 [0.35 to >100] kU A/L vs 1.53 [0.40-25.3] kU A/L; P=.0035). The prevalence of IgE reactivity to some allergens was higher in 1994 than in 1973, particularly rPhl p 5 (52% vs 19%), rPhl p 6 (43% vs 12%), and rBet v 1 (100% vs 29%). There was a correlation between timothy grass pollen-specific serum IgE levels and the numbers of IgE reactivities to individual allergens (p=0.76, P<.001).
The increase in specific IgE levels together with a possible increase in the prevalence of IgE reactivity to the major allergens Phl p 5 and Bet v 1 between 1973 and 1994 may have contributed to the increase in atopic conditions in Finland.
多项研究表明，近年来过敏和过敏原致敏的患病率有所上升。然而，针对个体过敏原的IgE反应模式的变化大多尚不清楚。
这项初步研究的目的是评估相隔20年随机收集的致敏个体血清中针对梯牧草和/或桦树花粉单个过敏原的IgE反应谱的变化。
从1973年和1994年在芬兰万马拉进行的2项横断面调查中，使用随机样本获得了51名致敏个体的血清样本。通过免疫测定法（免疫捕获法）分析血清对梯牧草和/或桦树花粉提取物、重组（r）Phl p 1、2、5、6、7、11、12、天然（n）Phl p 4以及rBet v 1、2和4的IgE反应性。
1994年针对梯牧草和桦树花粉的IgE抗体中位（范围）浓度高于1973年（6.47 [0.35至>100] kU A/L对1.53 [0.40 - 25.3] kU A/L；P = 0.0035）。1994年对某些过敏原的IgE反应性患病率高于1973年，尤其是rPhl p 5（52%对19%）、rPhl p 6（43%对12%）和rBet v 1（100%对29%）。梯牧草花粉特异性血清IgE水平与针对单个过敏原的IgE反应性数量之间存在相关性（p = 0.76，P < 0.001）。
1973年至1994年间，特异性IgE水平的升高以及对主要过敏原Phl p 5和Bet v 1的IgE反应性患病率可能增加，这可能导致了芬兰特应性疾病的增加。
