Qiu X-H, Bai W-Q, Zhong Q-Z, Li M, He F-Q, Li B-T
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
J Appl Microbiol. 2006 Nov;101(5):986-94. doi: 10.1111/j.1365-2672.2006.03016.x.
To isolate and characterize a methyl parathion (MP)-mineralizing bacterium, and to elucidate the degradative pathway of MP and localize the responsible degrading genes.
A bacterial strain, designated B2, capable of mineralizing MP was isolated from the MP-polluted soil. Analysis of the 16S rRNA gene sequence and phenotypic analysis suggested that strain B2 had a close relationship with Ochrobactrum anthropi. B2 could totally degrade MP and four metabolites [p-nitrophenol (PNP), 4-nitrocatechol (4-NC), 1,2,4-benzenetriol (BT) and hydroquinone (HQ)] were identified by HPLC and gas chromatography-mass spectrometry analyses. Plasmid curing of strain B2 resulted in the loss of ability of B2 to degrade PNP, but not the ability to hydrolyse MP.
Ochrobactrum sp. B2 can mineralize MP rapidly via PNP, 4-NC, BT and HQ pathway. B2 harbours a plasmid encoding the ability to degrade PNP, while MP-hydrolysing activity is encoded on the bacterial chromosome.
This new bacterial strain (B2) capable of mineralizing MP will be useful in a pure-culture remediation process of organophosphate pesticides and their metabolites such as nitroaromatics.
分离并鉴定一种能使甲基对硫磷（MP）矿化的细菌，阐明MP的降解途径并定位相关降解基因。
从受MP污染的土壤中分离出一株能够使MP矿化的细菌菌株，命名为B2。16S rRNA基因序列分析和表型分析表明，菌株B2与嗜人苍白杆菌关系密切。B2能够完全降解MP，通过高效液相色谱（HPLC）和气相色谱 - 质谱联用分析鉴定出四种代谢产物[对硝基苯酚（PNP）、4 - 硝基邻苯二酚（4 - NC）、1,2,4 - 苯三酚（BT）和对苯二酚（HQ）]。菌株B2的质粒消除导致其丧失降解PNP的能力，但不影响水解MP的能力。
嗜人苍白杆菌属B2菌株可通过PNP、4 - NC、BT和HQ途径快速使MP矿化。B2含有一个编码降解PNP能力的质粒，而MP水解活性由细菌染色体编码。
这种能够使MP矿化的新细菌菌株（B2）将有助于有机磷农药及其代谢产物（如硝基芳烃）的纯培养修复过程。
