Naess A, Sjursen H, Talstad I, Solberg C O
Medical Department B, University of Bergen, Haukeland Hospital, Norway.
APMIS. 1990 Dec;98(12):1135-8. doi: 10.1111/j.1699-0463.1990.tb05045.x.
Two gradient separation techniques were compared with the dextran sedimentation method for the separation of granulocytes from blood. Both techniques gave adequate yield and excellent purity, and flow cytometric analysis of surface membrane markers gave no indication of subset selection during the procedure. Cells separated by the three methods behaved comparably in functional tests including random migration, chemotaxis and chemiluminescence. The gradient separation techniques are rapid and efficient methods for the preparation of near 100% pure granulocyte suspensions for functional studies.
将两种梯度分离技术与葡聚糖沉降法进行比较，以从血液中分离粒细胞。两种技术均能提供足够的产量和优异的纯度，并且对表面膜标志物的流式细胞术分析未显示在该过程中有亚群选择的迹象。通过这三种方法分离的细胞在包括随机迁移、趋化性和化学发光在内的功能测试中表现相当。梯度分离技术是制备用于功能研究的近100%纯粒细胞悬液的快速有效方法。
