Hjorth R, Jonsson A K, Vretblad P
J Immunol Methods. 1981;43(1):95-101. doi: 10.1016/0022-1759(81)90040-5.
A simple one-step method for purification of human granulocytes, based on the use of a discontinuous gradient of Percoll, is described. The cell yield is 55% and the purity of the granulocyte fraction is 97%. The cells' ability to exclude trypan blue and phagocytic function are not altered during the purification process. We conclude that centrifugation in Percoll is superior to conventional dextran sedimentation; it is also less time-consuming.
本文描述了一种基于使用不连续 Percoll 梯度的简单一步法纯化人粒细胞的方法。细胞产量为 55%，粒细胞组分的纯度为 97%。在纯化过程中，细胞排除台盼蓝的能力和吞噬功能未发生改变。我们得出结论，Percoll 离心优于传统的葡聚糖沉降法；它也更省时。
