Schaible H G, Jarrott B, Hope P J, Duggan A W
Department of Preclinical Veterinary Sciences, University of Edinburgh, U.K.
Brain Res. 1990 Oct 8;529(1-2):214-23. doi: 10.1016/0006-8993(90)90830-5.
In anaesthetized spinal cats, the release of immunoreactive substance P in the spinal cord during development of an acute inflammation in one knee joint was studied with antibody microprobes. The microprobes bore antibodies directed to the C- or N-terminus of substance P. With the normal knee joint, innocuous mechanical stimuli (flexion, pressure) did not result in spinal release of immunoreactive substance P. Following injection of kaolin and carrageenan into a knee, evidence for release of substance P following joint stimulation was found in 7 of 10 cats. Such release did not occur for several hours after joint injection and was detected predominantly in the superficial dorsal horn, the dorsal columns and at the dorsal surface of the spinal cord. In some experiments release was detected in the deep dorsal horn and upper ventral horn. Release of immunoreactive substance P required periods of mechanical stimulation such as flexion of, or pressure to, the inflamed joint. The failure to detect central release of substance P from stimulation of normal joints, and the release of substance P, after a delay, from inflamed joints, suggest that the fibres releasing this compound require sensitization by inflammatory mediators before they are excited by joint stimuli.
在麻醉的脊髓猫中，用抗体微探针研究了一个膝关节急性炎症发展过程中脊髓内免疫反应性P物质的释放。微探针带有针对P物质C端或N端的抗体。对于正常膝关节，无害的机械刺激（屈曲、按压）不会导致脊髓释放免疫反应性P物质。在向膝关节注射高岭土和角叉菜胶后，10只猫中有7只在关节刺激后出现了P物质释放的证据。这种释放在关节注射后数小时内不会发生，且主要在脊髓背角浅层、背柱和脊髓背表面检测到。在一些实验中，在脊髓背角深层和腹角上部也检测到了释放。免疫反应性P物质的释放需要对发炎关节进行诸如屈曲或按压等机械刺激。未能从正常关节刺激中检测到P物质的中枢释放，以及在发炎关节刺激后延迟释放P物质，这表明释放这种化合物的纤维在被关节刺激激发之前需要炎症介质的致敏作用。
