Hope P J, Jarrott B, Schaible H G, Clarke R W, Duggan A W
Department of Preclinical Veterinary Sciences, University of Edinburgh, Summerhall, U.K.
Brain Res. 1990 Nov 19;533(2):292-9. doi: 10.1016/0006-8993(90)91352-h.
Antibody microprobes were used to study the release of immunoreactive neurokinin A into the spinal cord of anaesthetised cats during and following injection of a knee joint with kaolin and carrageenan. A basal level of immunoreactive neurokinin A was detected prior to any noxious stimuli. Innocuous mechanical joint stimuli (flexion or pressure) did not alter this basal level of release. However, on injection of kaolin and carrageenan into a knee joint, evidence of release into the ipsilateral spinal cord was immediately observed. Initially, immunoreactive neurokinin A was detected in 2 regions: one at the dorsal surface of the spinal cord and the other centred on the superficial dorsal horn. Within 1 h of joint injection, however, immunoreactive neurokinin A was detected throughout the dorsal horn and the adjacent white matter. The extensive spread and persistence of immunoreactive neurokinin A in the spinal cord may underlie some of the prolonged excitability changes evoked by brief noxious stimuli and peripheral inflammation reported by other laboratories.
抗体微探针被用于研究在向麻醉猫的膝关节注射高岭土和角叉菜胶期间及之后，免疫反应性神经激肽A释放到脊髓中的情况。在施加任何有害刺激之前，检测到了免疫反应性神经激肽A的基础水平。无害的机械性关节刺激（屈曲或按压）并未改变这种基础释放水平。然而，在向膝关节注射高岭土和角叉菜胶后，立即观察到了向同侧脊髓释放的迹象。最初，在两个区域检测到了免疫反应性神经激肽A：一个在脊髓背表面，另一个以浅背角为中心。然而，在关节注射后1小时内，在整个背角和相邻白质中都检测到了免疫反应性神经激肽A。免疫反应性神经激肽A在脊髓中的广泛扩散和持续存在，可能是其他实验室报道的短暂有害刺激和外周炎症所诱发的一些长时间兴奋性变化的基础。
