Duraipandiyan Veeramuthu, Ayyanar Muniappan, Ignacimuthu Savarimuthu
Entomology Research Institute, Loyola College, Chennai, India.
BMC Complement Altern Med. 2006 Oct 17;6:35. doi: 10.1186/1472-6882-6-35.
Antimicrobial activity of 18 ethnomedicinal plant extracts were evaluated against nine bacterial strains (Bacillus subtilis, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Ervinia sp, Proteus vulgaris) and one fungal strain (Candida albicans). The collected ethnomedicinal plants were used in folk medicine in the treatment of skin diseases, venereal diseases, respiratory problems and nervous disorders.
Plants were collected from Palni hills of Southern Western Ghats and the ethnobotanical data were gathered from traditional healers who inhabit the study area. The hexane and methanol extracts were obtained by cold percolation method and the antimicrobial activity was found using paper disc diffusion method. All microorganisms were obtained from Christian Medical College, Vellore, Tamil Nadu, India.
The results indicated that out of 18 plants, 10 plants exhibited antimicrobial activity against one or more of the tested microorganisms at three different concentrations of 1.25, 2.5 and 5 mg/disc. Among the plants tested, Acalypha fruticosa, Peltophorum pterocarpum, Toddalia asiatica,Cassia auriculata, Punica granatum and Syzygium lineare were most active. The highest antifungal activity was exhibited by methanol extract of Peltophorum pterocarpum and Punica granatum against Candida albicans.
This study evaluated the antimicrobial activity of the some ethnomedicinal plants used in folkloric medicine. Compared to hexane extract, methanol extract showed significant activity against tested organisms. This study also showed that Toddalia asiatica, Syzygium lineare, Acalypha fruticosa and Peltophorum pterocarpum could be potential sources of new antimicrobial agents.
评估了18种民族药用植物提取物对9种细菌菌株（枯草芽孢杆菌、金黄色葡萄球菌、表皮葡萄球菌、粪肠球菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌、欧文氏菌属、普通变形杆菌）和1种真菌菌株（白色念珠菌）的抗菌活性。所收集的民族药用植物在民间医学中用于治疗皮肤病、性病、呼吸道问题和神经紊乱。
从西高止山脉南部的帕尔尼山采集植物，并从居住在研究区域的传统治疗师那里收集民族植物学数据。通过冷渗滤法获得己烷和甲醇提取物，并使用纸片扩散法测定抗菌活性。所有微生物均取自印度泰米尔纳德邦韦洛尔的基督教医学院。
结果表明，在18种植物中，有10种植物在1.25、2.5和5mg/片这三种不同浓度下对一种或多种测试微生物表现出抗菌活性。在所测试的植物中，灌木铁苋菜、盾柱木、飞龙掌血、决明、石榴和线叶蒲桃最为活跃。盾柱木和石榴的甲醇提取物对白色念珠菌表现出最高的抗真菌活性。
本研究评估了一些民间医学中使用的民族药用植物的抗菌活性。与己烷提取物相比，甲醇提取物对测试生物体表现出显著活性。本研究还表明，飞龙掌血、线叶蒲桃、灌木铁苋菜和盾柱木可能是新型抗菌剂的潜在来源。
