Development of sensitive methods for the detection of mycobacteria by DNA probes.
作者信息
Rahav G, Sela S, Bercovier H
机构信息
Department of Clinical Microbiology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
出版信息
FEMS Microbiol Lett. 1990 Oct;60(1-2):29-33. doi: 10.1016/0378-1097(90)90339-r.
PMID:1704336
Abstract
A sensitive method allowing the detection of mycobacteria by DNA probing has been developed. Besides the choice of a relevant probe encoding for part of the ribosomal RNA genes, the critical step allowing this high sensitivity was the method by which mycobacteria were lysed. Sonication of mycobacteria in the presence of chloroform followed by dot blot by this lysate gave the highest sensitivity in the detection of sequences homologous to the DNA probes.
摘要
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